An antifouling electrochemical aptasensor based on a Y-shaped peptide for tetracycline detection in milk
文献类型：期刊论文
| 作者 | Pu, Xujun2; Hu, Yuanling2; Niu, Meirong2; Liu, Hongcheng4; Li, Chenguo1,3; Ma, Wenlong3 ; Gu, Ying2
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| 刊名 | JOURNAL OF FOOD COMPOSITION AND ANALYSIS
 |
| 出版日期 | 2024-08-01 |
| 卷号 | 132页码:10 |
| 关键词 | Antifouling Peptide Electrochemical aptasensor Tetracycline Protein Food safety |
| ISSN号 | 0889-1575 |
| DOI | 10.1016/j.jfca.2024.106349 |
| 通讯作者 | Ma, Wenlong(mawenlong@qdio.ac.cn) ; Gu, Ying(guying@kust.edu.cn) |
| 英文摘要 | Nonspecific adsorption of non-target components (especially proteins) in food matrix on sensing surfaces deteriorates accuracy and sensitivity of electrochemical sensors, which is a key challenge for food safety sensing. Therefore, electrochemical sensors with antifouling capability of high-protein food matrices are highly desired. In this paper, an efficient and simple antifouling electrochemical aptasensor for tetracycline (TC) analysis was constructed based on a self-designed Y-shaped peptide [CPPPPEK-(RSERSERSE)2] consisting of cysteine as the anchoring segment, polyproline as the supporting segment, and -EK-(RSERSERSE)2 as antifouling branches. Hydrophilic and electrically neutral amino acid sequence and Y-shaped space structure of the peptide endow the sensing surface with good antifouling performance in protein solutions and diluted milk. Under optimized experiment conditions, the proposed aptasensor can detect TC with a wide linear range (0.01-100 ng mL-1) and a limit of detection of 5.3 pg mL-1 (S/N = 3). The spiked recovery experiments confirmed high accuracy of the aptasensor in diluted milk without other pretreatments (recoveries: 90.96%-95.86%). This strategy offers a valid way to reduce matrix interference, showing a promise to be extended to other sensing systems for different targets. |
| WOS关键词 | ZWITTERIONIC PEPTIDE ; PERFORMANCE |
| 资助项目 | Yunnan Major Scientific and Tech- nological Projects[202202AG050009] ; National Natural Science Foundation of China[32102073] ; Yunnan Funda- mental Research Projects[202201AU070106] ; Yunnan Funda- mental Research Projects[202101BE070001-052] ; Special Project for High-level Scientific and Technological Talents and Innovation Teams of Yunnan Province[202405AS350005] |
| WOS研究方向 | Chemistry ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:001245029600001 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186554]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Ma, Wenlong; Gu, Ying |
| 作者单位 | 1.China Univ Petr, Coll Mech & Elect Engn, Qingdao 266580, Peoples R China 2.Kunming Univ Sci & Technol, Fac Food Sci & Engn, Kunming 650500, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Yunnan Acad Agr Sci, Qual Stand & Testing Technol Res Inst, Kunming 650205, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pu, Xujun,Hu, Yuanling,Niu, Meirong,et al. An antifouling electrochemical aptasensor based on a Y-shaped peptide for tetracycline detection in milk[J]. JOURNAL OF FOOD COMPOSITION AND ANALYSIS,2024,132:10. |
| APA | Pu, Xujun.,Hu, Yuanling.,Niu, Meirong.,Liu, Hongcheng.,Li, Chenguo.,...&Gu, Ying.(2024).An antifouling electrochemical aptasensor based on a Y-shaped peptide for tetracycline detection in milk.JOURNAL OF FOOD COMPOSITION AND ANALYSIS,132,10. |
| MLA | Pu, Xujun,et al."An antifouling electrochemical aptasensor based on a Y-shaped peptide for tetracycline detection in milk".JOURNAL OF FOOD COMPOSITION AND ANALYSIS 132(2024):10. |
入库方式： OAI收割
来源：海洋研究所
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