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Screening for Aspergillus fumigatus strain-2T-2 with high chitosanase production activity and its application in chitosan degradation q

文献类型：期刊论文

作者Yang, Haoyue1,2; Wang, Linsong1,2; Xu, Chaojie1,2; Hao, Wentong1,2; Xing, Ronge1,2; Liu, Song1,2; Yu, Huahua1,2; Li, Pengcheng2
刊名ELECTRONIC JOURNAL OF BIOTECHNOLOGY
出版日期2024-03-01
卷号68页码:57-66
关键词Aspergillus fumigatus strain-2T-2 Aspergillus fumigatus Chitooligosaccharide Chitosan Chitosanase Chitosanase-producing strain Degradation Fumigatus chitosanase MH7A cells
ISSN号0717-3458
DOI10.1016/j.ejbt.2024.01.003
通讯作者Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要Background: High chitosanase-producing microorganisms from natural sources have extensive applications in food and agriculture. This study aimed to optimal conditions for high-activity chitosanase production. A named CGMCC21422 chitosanase-producing strain -2T-2 was isolated from soil and identified named as Aspergillus fumigatus chitosanase (A. fumigatus chitosanase). This enzymatic activity was validated in various culture conditions. It is stored in the China General Microbiological Culture Collection Center. The efficacy of A. fumigatus chitosanase in the degradation of chitosan was validated. Results: In this study, we determined that the optimal fermentation conditions of stain-2T-2 were 1.0% powered chitosan, 0.8% ammonium nitrate, 37 degrees C culture temperature, initial pH 5.0, culture time 6 d, bottle volume 50 mL, and 2% inoculation dosage. Under these culture conditions, the highest enzyme activity of fermentation broth in the shaker flask reached 827.53 U/mL. The optimal reactive conditions of A. fumigatus-produced chitosanase are 55-60 degrees C and pH 4.5. When the reactive temperature was over 60 degrees C, the A. fumigatus chitosanase was easily inactivated. The chitosanase catalyzed substrate chitosan to produced X20% chito-oligosaccharide and >= 80% glucosamine salt samples in a variety of acidic solutions. These reactive products are not cytotoxic or mild to MH7A cells. Conclusions: A. fumigatus chitosanase strain -2T-2 is a strain with high chitosanase and can catalyze chitosan into chito-oligosaccharide in acidic solutions. How to cite: Yang, H., Wang, L., Xu, C, et al. Screening for Aspergillus fumigatus strain-2T-2 with high chitosanase production activity and its application in chitosan degradation. Electron J Biotechnol 2024; 68. https://doi.org/10.1016/j.ejbt.2024.01.003. (c) 2023 The Authors. Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B. V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).
WOS关键词CRYSTAL-STRUCTURE ; PURIFICATION ; OLIGOSACCHARIDES
资助项目Key R&D Program (Major Scientific and Technological Innovation Project) of Shandong Province, China[2022CXGC020413] ; Key R&D Program (Major Scientific and Technological Innovation Project) of Shandong Province, China[2023CXGC010412] ; Natural Science Foundation of Shandong Province[ZR2022YQ37] ; International Partnership Program of Chinese Academy of Sciences[058GJHZ2023044FN]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:001205515500001
出版者PONTIFICIA UNIV CATOLICA VALPARAISO
源URL[http://ir.qdio.ac.cn/handle/337002/185561]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Xing, Ronge; Li, Pengcheng
作者单位1.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266237, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Haoyue,Wang, Linsong,Xu, Chaojie,et al. Screening for Aspergillus fumigatus strain-2T-2 with high chitosanase production activity and its application in chitosan degradation q[J]. ELECTRONIC JOURNAL OF BIOTECHNOLOGY,2024,68:57-66.
APA Yang, Haoyue.,Wang, Linsong.,Xu, Chaojie.,Hao, Wentong.,Xing, Ronge.,...&Li, Pengcheng.(2024).Screening for Aspergillus fumigatus strain-2T-2 with high chitosanase production activity and its application in chitosan degradation q.ELECTRONIC JOURNAL OF BIOTECHNOLOGY,68,57-66.
MLA Yang, Haoyue,et al."Screening for Aspergillus fumigatus strain-2T-2 with high chitosanase production activity and its application in chitosan degradation q".ELECTRONIC JOURNAL OF BIOTECHNOLOGY 68(2024):57-66.

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来源：海洋研究所

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