中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 海洋研究所
  3. 中国科学院海洋研究所
  4. 实验海洋生物学重点实验室
The combination of high temperature and Vibrio infection worsens summer mortality in the clam Meretrix petechialis by increasing apoptosis and oxidative stress

文献类型：期刊论文

作者Tian, Jing1,2,5; Wang, Di1,2,5; Wang, Hongxia1,2,3; Huan, Pin1,2,3,6; Liu, Baozhong1,2,3,4,6
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2024-06-01
卷号149页码:10
关键词Vibrio Heat stress Immune response Transcriptome Meretrix petechialis
ISSN号1050-4648
DOI10.1016/j.fsi.2024.109542
通讯作者Liu, Baozhong(bzliu@qdio.ac.cn)
英文摘要The interaction between environmental factors and Vibrio in bivalves is not well understood, despite the widely held belief that pathogen infection and seawater temperature significantly impact summer mortality. In the present study, we conducted simulated experiments to explore the effects of high temperature and Vibrio infection on the clam Meretrix petechialis. The survival curve analysis revealed that the combined challenge of high temperature and Vibrio infection (31 degrees C-vibrio) led to significantly higher clam mortality compared to the groups exposed solely to Vibrio (27 degrees C-vibrio), high temperature (31 degrees C-control), and the control condition (27 degrees Ccontrol). Furthermore, PCoA analysis of 11 immune genes indicated that Vibrio infection predominated during the incubation period, with a gradual equilibrium between these factors emerging during the course of the infection. Additionally, our investigations into apoptosis and autophagy processes exhibited significant induction of mTOR and Bcl2 of the 31 degrees C-vibrio group in the early challenge stage, followed by inhibition in the later stage. Oxidative stress analysis demonstrated a substantial additive effect on malondialdehyde (MDA) and glutathione (GSH) content in the combined challenge group compared to the control group. Comparative transcriptome analysis revealed a significant increase in differentially expressed genes related to immunity, such as complement C1q-like protein, C-type lectin, big defensin, and lysozyme, in the 31 degrees C-vibrio group, suggesting that the synergistic effect of high temperature and Vibrio infection triggers more robust antibacterial immune responses. These findings provide critical insights for understanding the infection process and uncovering the causes of summer mortality.
WOS关键词ENERGY-METABOLISM ; CRASSOSTREA-GIGAS ; MASS MORTALITIES ; PACIFIC OYSTER ; IMMUNE ; SPLENDIDUS ; EXPRESSION ; CHALLENGE ; PATHOGENS ; RESPONSES
资助项目Oceanographic Data Center, Institute of Oceanology, Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030304] ; Key Research and Development Program of Shandong[2021LZGC029] ; Earmarked fund for CARS-49 ; Taishan Industrial Leading Talents Project
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:001223643200001
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
源URL[http://ir.qdio.ac.cn/handle/337002/185769]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Liu, Baozhong
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Tian, Jing,Wang, Di,Wang, Hongxia,et al. The combination of high temperature and Vibrio infection worsens summer mortality in the clam Meretrix petechialis by increasing apoptosis and oxidative stress[J]. FISH & SHELLFISH IMMUNOLOGY,2024,149:10.
APA Tian, Jing,Wang, Di,Wang, Hongxia,Huan, Pin,&Liu, Baozhong.(2024).The combination of high temperature and Vibrio infection worsens summer mortality in the clam Meretrix petechialis by increasing apoptosis and oxidative stress.FISH & SHELLFISH IMMUNOLOGY,149,10.
MLA Tian, Jing,et al."The combination of high temperature and Vibrio infection worsens summer mortality in the clam Meretrix petechialis by increasing apoptosis and oxidative stress".FISH & SHELLFISH IMMUNOLOGY 149(2024):10.

入库方式： OAI收割

来源：海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。