Characterization of the Apoptotic and Antimicrobial Activities of Two Initiator Caspases of Sea Cucumber Apostichopus japonicus
文献类型：期刊论文
| 作者 | Zhu, Hanshuo1,2,3 ; Yuan, Zihao1,2,3; Xu, Hang1; Sun, Li1,2,3
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| 刊名 | GENES
 |
| 出版日期 | 2024-05-01 |
| 卷号 | 15期号:5页码:13 |
| 关键词 | Apostichopus japonicus caspase apoptosis antimicrobial |
| DOI | 10.3390/genes15050540 |
| 通讯作者 | Xu, Hang(xuhang@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | Caspase (CASP) is a protease family that plays a vital role in apoptosis, development, and immune response. Herein, we reported the identification and characterization of two CASPs, AjCASPX1 and AjCASPX2, from the sea cucumber Apostichopus japonicus, an important aquaculture species. AjCASPX1/2 share similar domain organizations with the vertebrate initiator caspases CASP2/9, including the CARD domain and the p20/p10 subunits with conserved functional motifs. However, compared with human CASP2/9, AjCASPX1/2 possess unique structural features in the linker region between p20 and p10. AjCASPX1, but not AjCASPX2, induced marked apoptosis of human cells by activating CASP3/7. The recombinant proteins of AjCASPX2 and the CARD domain of AjCASPX2 were able to bind to a wide range of bacteria, as well as bacterial cell wall components, and inhibit bacterial growth. AjCASPX1, when expressed in Escherichia coli, was able to kill the host bacteria. Under normal conditions, AjCASPX1 and AjCASPX2 expressions were most abundant in sea cucumber muscle and coelomocytes, respectively. After bacterial infection, both AjCASPX1 and AjCASPX2 expressions were significantly upregulated in sea cucumber tissues and cells. Together, these results indicated that AjCASPX1 and AjCASPX2 were initiator caspases with antimicrobial activity and likely functioned in apoptosis and immune defense against pathogen infection. |
| WOS关键词 | ACTIVATION ; CELOMOCYTES ; MECHANISMS ; AUTOLYSIS |
| 资助项目 | Science & Technology Innovation Project of Laoshan Laboratory |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:001232384200001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/185936]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Xu, Hang; Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266404, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Coll Marine Sci, Qingdao 266404, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Hanshuo,Yuan, Zihao,Xu, Hang,et al. Characterization of the Apoptotic and Antimicrobial Activities of Two Initiator Caspases of Sea Cucumber Apostichopus japonicus[J]. GENES,2024,15(5):13. |
| APA | Zhu, Hanshuo,Yuan, Zihao,Xu, Hang,&Sun, Li.(2024).Characterization of the Apoptotic and Antimicrobial Activities of Two Initiator Caspases of Sea Cucumber Apostichopus japonicus.GENES,15(5),13. |
| MLA | Zhu, Hanshuo,et al."Characterization of the Apoptotic and Antimicrobial Activities of Two Initiator Caspases of Sea Cucumber Apostichopus japonicus".GENES 15.5(2024):13. |
入库方式： OAI收割
来源：海洋研究所
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