Optimization of the Preparation Process of Glucuronomannan Oligosaccharides and Their Effects on the Gut Microbiota in MPTP-Induced PD Model Mice
文献类型：期刊论文
| 作者 | Wang, Baoxiang1,4,5; Geng, Lihua2,4,5,6; Wang, Jing2,4,5,6 ; Wei, Yuxi1; Yan, Changhui3,4,5; Wu, Ning2,4,5; Yue, Yang2,4,5,6; Zhang, Quanbin2,4,5,6
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| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2024-05-01 |
| 卷号 | 22期号:5页码:19 |
| 关键词 | glucuronomannan oligosaccharide Parkinson's disease (PD) gut microbiota |
| DOI | 10.3390/md22050193 |
| 通讯作者 | Geng, Lihua(lhgeng@qdio.ac.cn) ; Zhang, Quanbin(qbzhang@qdio.ac.cn) |
| 英文摘要 | Parkinson's disease (PD) is a prevalent neurodegenerative disorder, and accumulating evidence suggests a link between dysbiosis of the gut microbiota and the onset and progression of PD. In our previous investigations, we discovered that intraperitoneal administration of glucuronomannan oligosaccharides (GMn) derived from Saccharina japonica exhibited neuroprotective effects in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mouse model. However, the complicated preparation process, difficulties in isolation, and remarkably low yield have constrained further exploration of GMn. In this study, we optimized the degradation conditions in the preparation process of GMn through orthogonal experiments. Subsequently, an MPTP-induced PD model was established, followed by oral administration of GMn. Through a stepwise optimization, we successfully increased the yield of GMn, separated from crude fucoidan, from 1 similar to 2/10,000 to 4 similar to 8/1000 and indicated the effects on the amelioration of MPTP-induced motor deficits, preservation of dopamine neurons, and elevation in striatal neurotransmitter levels. Importantly, GMn mitigated gut microbiota dysbiosis induced by MPTP in mice. In particular, GM2 significantly reduced the levels of Akkermansia, Verrucomicrobiota, and Lactobacillus, while promoting the abundance of Roseburia and Prevotella compared to the model group. These findings suggest that GM2 can potentially suppress PD by modulating the gut microbiota, providing a foundation for the development of a novel and effective anti-PD marine drug. |
| WOS关键词 | PARKINSONS-DISEASE ; SACCHARINA-JAPONICA ; FUCOIDAN PROTECTS ; HYPERBARIC-OXYGEN ; RAT MODEL ; NEUROINFLAMMATION |
| 资助项目 | Shandong Provincial Natural Science Foundation |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001233477000001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/185957]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Geng, Lihua; Zhang, Quanbin |
| 作者单位 | 1.Qingdao Univ, Coll Life Sci, 308 Ningxia Rd, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 3.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Key Lab Opt Elect Sensing & Analyt Chem Life Sci, MOE, Qingdao 266042, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 6.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Baoxiang,Geng, Lihua,Wang, Jing,et al. Optimization of the Preparation Process of Glucuronomannan Oligosaccharides and Their Effects on the Gut Microbiota in MPTP-Induced PD Model Mice[J]. MARINE DRUGS,2024,22(5):19. |
| APA | Wang, Baoxiang.,Geng, Lihua.,Wang, Jing.,Wei, Yuxi.,Yan, Changhui.,...&Zhang, Quanbin.(2024).Optimization of the Preparation Process of Glucuronomannan Oligosaccharides and Their Effects on the Gut Microbiota in MPTP-Induced PD Model Mice.MARINE DRUGS,22(5),19. |
| MLA | Wang, Baoxiang,et al."Optimization of the Preparation Process of Glucuronomannan Oligosaccharides and Their Effects on the Gut Microbiota in MPTP-Induced PD Model Mice".MARINE DRUGS 22.5(2024):19. |
入库方式： OAI收割
来源：海洋研究所
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