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Different Immune Responses of Hemocytes from V. parahaemolyticus-Resistant and -Susceptible Shrimp at Early Infection Stage

文献类型：期刊论文

作者Du, Wenran1,2,3,4; Li, Shihao1,3,4,5,6; Li, Fuhua1,3,4,5,6
刊名BIOLOGY-BASEL
出版日期2024-05-01
卷号13期号:5页码:12
关键词penaeid shrimp hemocytes Vibrio parahaemolyticus glycolysis immunity
DOI10.3390/biology13050300
通讯作者Li, Shihao(lishihao@qdio.ac.cn)
英文摘要Vibrio parahaemolyticus is one of the main causative agents leading to acute hepatopancreatic necrosis disease, the severe bacterial disease that occurs during shrimp aquaculture. Hemocytes play important roles during Vibrio infection. Previously, we found that there were few differentially expressed genes (DEGs) between hemocytes from V. parahaemolyticus-resistant and -susceptible shrimp before infection. We considered that there should be different immune responses between them after a pathogen infection. Here, the transcriptome data of hemocytes from V. parahaemolyticus-resistant and -susceptible shrimp before and after a pathogen infection were compared. The results showed that there were 157 DEGs responsive to infection in V. parahaemolyticus-resistant shrimp, while 33 DEGs in V. parahaemolyticus-susceptible shrimp. DEGs in V. parahaemolyticus-resistant shrimp were mainly related to immune and glycolytic processes, while those in V. parahaemolyticus-susceptible shrimp were mainly related to metabolism, with only two DEGs in common. A further analysis of genes involved in glucose metabolism revealed that GLUT2, HK, FBP, and PCK1 were lowly expressed while PC were highly expressed in hemocytes of the V. parahaemolyticus-resistant shrimp, indicating that glucose metabolism in shrimp hemocytes was related to a V. parahaemolyticus infection. After the knockdown of PC, the expression of genes in Toll and IMD signaling pathways were down-regulated, indicating that glucose metabolism might function through regulating host immunity during V. parahaemolyticus infection. The results suggest that the immune responses between V. parahaemolyticus-resistant and -susceptible shrimp were apparently different, which probably contribute to their different V. parahaemolyticus resistance abilities.
WOS关键词EXPRESSION ; TREHALOSE ; STRINGTIE ; HISAT
资助项目National Natural Science Foundation of China
WOS研究方向Life Sciences & Biomedicine - Other Topics
语种英语
WOS记录号WOS:001233527400001
出版者MDPI
源URL[http://ir.qdio.ac.cn/handle/337002/185972]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Li, Shihao
作者单位1.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
2.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China
3.Chinese Acad Sci, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China
6.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Du, Wenran,Li, Shihao,Li, Fuhua. Different Immune Responses of Hemocytes from V. parahaemolyticus-Resistant and -Susceptible Shrimp at Early Infection Stage[J]. BIOLOGY-BASEL,2024,13(5):12.
APA Du, Wenran,Li, Shihao,&Li, Fuhua.(2024).Different Immune Responses of Hemocytes from V. parahaemolyticus-Resistant and -Susceptible Shrimp at Early Infection Stage.BIOLOGY-BASEL,13(5),12.
MLA Du, Wenran,et al."Different Immune Responses of Hemocytes from V. parahaemolyticus-Resistant and -Susceptible Shrimp at Early Infection Stage".BIOLOGY-BASEL 13.5(2024):12.

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来源：海洋研究所

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