Sodium selenite enhances the production of functional proteins and biomass in Chlorella pyrenoidosa 038F by promoting acetate assimilation under heterotrophic cultivation
文献类型：期刊论文
| 作者 | Shangguan, Jingjing3,4,5; Yang, Na3,4; Zhang, Litao3,4; Liu, Jianguo2,3,4; Li, Yueming1; Xu, Jianchun1; Xia, Xiuluan1; Xu, Bingzheng1 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2024-08-30 |
| 卷号 | 589页码:10 |
| 关键词 | Glutathione peroxidase Chlorella pyrenoidosa Sodium selenite Carbon metabolism Transcriptome Gluconeogenesis |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2024.740987 |
| 通讯作者 | Liu, Jianguo(jgliu@qdio.ac.cn) |
| 英文摘要 | Unicellular microalga Chlorella pyrenoidosa is an excellent source of protein, but its ability to produce functional proteins has received less attention. In this study, a strategy to enhance the levels of functional proteins and biomass, by adding sodium selenite under heterotrophic cultivation, is reported for C. pyrenoidosa 038F. Activities of catalase, superoxide dismutase, and glutathione peroxidase were significantly enhanced by 1.94-, 1.90and 32.73-fold, respectively, in C. pyrenoidosa 038F exposed to 20 mg/L of sodium selenite. Further, western blot analysis confirmed that sodium selenite promoted the synthesis of glutathione peroxidase, which might be due to the up-regulation of peroxisome and selenocysteine-synthesis-related genes. Sodium selenite also increased biomass, the content of protein and soluble sugar by 1.32-, 1.29-, 1.46-fold, respectively. Subsequent transcriptomic analysis confirmed that cellular respiration and the gluconeogenesis pathway were enhanced. This study highlights the potential use of heterotrophic C. pyrenoidosa 038F as a novel selenoprotein-rich carrier while simultaneously producing soluble sugar and biomass. These results might be helpful for increasing economic production of C. pyrenoidosa under heterotrophic cultivation. |
| WOS关键词 | GROWTH ; RESPIRATION ; STRESS ; PLANTS |
| 资助项目 | TS Industrial Experts Programme[LJNY202102] ; Strategic Priority Research Program of the Chinese Academy of Sciences, China[XDB42000000] ; Science & Technology Specific Project in Agricultural High-tech Industrial Demonstration Area of the Yellow River Delta[2022SZX13] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001235420600001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/185987]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Jianguo |
| 作者单位 | 1.Qingdao Langyatai Grp Co Ltd, Qingdao 266500, Peoples R China 2.Natl Ctr Technol Innovat Comprehens Utilizat Salin, Acad Workstat Agr High Tech Ind Area Yellow River, Dongying 257300, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, 168 Wenhai Rd, Qingdao 266237, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shangguan, Jingjing,Yang, Na,Zhang, Litao,et al. Sodium selenite enhances the production of functional proteins and biomass in Chlorella pyrenoidosa 038F by promoting acetate assimilation under heterotrophic cultivation[J]. AQUACULTURE,2024,589:10. |
| APA | Shangguan, Jingjing.,Yang, Na.,Zhang, Litao.,Liu, Jianguo.,Li, Yueming.,...&Xu, Bingzheng.(2024).Sodium selenite enhances the production of functional proteins and biomass in Chlorella pyrenoidosa 038F by promoting acetate assimilation under heterotrophic cultivation.AQUACULTURE,589,10. |
| MLA | Shangguan, Jingjing,et al."Sodium selenite enhances the production of functional proteins and biomass in Chlorella pyrenoidosa 038F by promoting acetate assimilation under heterotrophic cultivation".AQUACULTURE 589(2024):10. |
入库方式： OAI收割
来源：海洋研究所
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