Gene structure, expression and function analysis of the MyoD gene in the Pacific white shrimp Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Xia, Yanting1,2,3; Zhang, Xiaojun1,2,3,5,6; Zhang, Xiaoxi1,3 ; Zhu, Haochen1,2,3; Zhong, Xiaoyun1,3,6; Song, Weixiao4; Yuan, Jianbo1,3,5,6; Sha, Zhenxia2; Li, Fuhua1,3,5,6
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| 刊名 | GENE
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| 出版日期 | 2024-08-30 |
| 卷号 | 921页码:14 |
| 关键词 | MyoD gene Litopenaeus vannamei RNA interference Expression Growth |
| ISSN号 | 0378-1119 |
| DOI | 10.1016/j.gene.2024.148523 |
| 通讯作者 | Zhang, Xiaojun(xjzhang@qdio.ac.cn) |
| 英文摘要 | The Pacific white shrimp Litopenaeus vannamei is a representative species of decapod crustacean and an economically important marine aquaculture species worldwide. However, research on the genes involved in muscle growth and development in shrimp is still lacking. MyoD is recognized as a crucial regulator of myogenesis and plays an essential role in muscle growth and differentiation in various animals. Nonetheless, little information is available concerning the function of this gene among crustaceans. In this study, we identified a sequence of the MyoD gene (LvMyoD) with a conserved bHLH domain in the L. vannamei genome. Phylogenetic analysis revealed that both the overall protein sequence and specific functional sites of LvMyoD are highly conserved with those of other crustacean species and that they are evolutionarily closely related to vertebrate MyoD and Myf5. LvMyoD expression is initially high during early muscle development in shrimp and gradually decreases after 40 days post-larval development. In adults, the muscle-specific expression of LvMyoD was confirmed through RT-qPCR analysis. Knockdown of LvMyoD inhibited the growth of the shrimp in body length and weight. Histological observation and transcriptome sequencing of muscle samples after RNA interference (RNAi) revealed nuclear agglutination and looseness in muscle fibers. Additionally, we observed significant effects on the expression of genes involved in heat shock proteins, myosins, actins, protein synthesis, and glucose metabolism. These findings suggest that LvMyoD plays a critical role in regulating muscle protein synthesis and muscle cell differentiation. Overall, this study highlights the involvement of LvMyoD in myogenesis and muscle growth, suggesting that it is a potentially important regulatory target for shrimp breeding efforts. |
| WOS关键词 | HEAT-SHOCK PROTEINS ; MUSCLE DIFFERENTIATION ; RYANODINE RECEPTORS ; MYOGENIC FACTOR ; TRANSCRIPTION ; DROSOPHILA ; FAMILY ; FIBROBLASTS ; NAUTILUS ; CELLS |
| 资助项目 | National Natural Sciences Foundation of China[32273102] ; National Key R &D Program of China[2022YFF1000304] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105] ; Taishan Scholars Program ; Oceanographic Data Center, IOCAS |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:001240765500001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186011]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, Xiaojun |
| 作者单位 | 1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Qingdao Univ, Inst Aquat Biotechnol, Collage Life Sci, Qingdao 266071, Peoples R China 3.Inst Oceanol, Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Univ British Columbia, Vancouver, BC, Canada 5.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China 6.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Yanting,Zhang, Xiaojun,Zhang, Xiaoxi,et al. Gene structure, expression and function analysis of the MyoD gene in the Pacific white shrimp Litopenaeus vannamei[J]. GENE,2024,921:14. |
| APA | Xia, Yanting.,Zhang, Xiaojun.,Zhang, Xiaoxi.,Zhu, Haochen.,Zhong, Xiaoyun.,...&Li, Fuhua.(2024).Gene structure, expression and function analysis of the MyoD gene in the Pacific white shrimp Litopenaeus vannamei.GENE,921,14. |
| MLA | Xia, Yanting,et al."Gene structure, expression and function analysis of the MyoD gene in the Pacific white shrimp Litopenaeus vannamei".GENE 921(2024):14. |
入库方式： OAI收割
来源：海洋研究所
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