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Expression characteristics and interference analysis of glucose-6-phosphate dehydrogenase reveal its importance in Pyropia yezoensis under salt stress

文献类型：期刊论文

作者Shi, Mengmeng4,5; Lin, Guohong4,5; Zhang, Baoyu1,2,5; Xie, Xiujun1,2,5; Qiang, Xi2,5; Niu, Jianfeng1,2,5; Zheng, Zhenbing1,2,5; Wang, Xulei1,2,5; Wang, Guangce1,2,3,5
刊名ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
出版日期2024-06-01
卷号80页码:7
关键词Cytoplasmic G6PDH Chloroplastic G6PDH Pyropia yezoensis Salt stress Cyclic electron flow
ISSN号2211-9264
DOI10.1016/j.algal.2024.103522
通讯作者Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要Glucose -6 -phosphate dehydrogenase (G6PDH) is a key enzyme in the pentose phosphate pathway and plays a crucial role in photosynthetic organisms responding to stress. In this study, we analyzed the bioinformatics of enzymes involved in the oxidative pentose phosphate pathway in Pyropia yezoensis and examined the expression of corresponding genes under salt stress. The results showed that the presence of G6PDH and 6-phosphogluconate dehydrogenase in both the cytoplasm and chloroplasts of P. yezoensis , with upregulation of both enzyme genes in the cytoplasm and downregulation in the chloroplasts under high salt stress. Mutant strains with silenced cytoplasmic and plastidic G6PDH showed significantly reduced photosynthetic activity and restricted cyclic electron flow compared to the wild type under high salt stress, suggesting that interference with G6PDH weakened Pyropia 's response to high salt stress. This study clarifies Pyropia 's response to salt stress through the oxidative pentose phosphate pathway, and lays a theoretical foundation for deciphering the stress response mechanism of G6PDH.
WOS关键词PENTOSE-PHOSPHATE PATHWAY ; CYCLIC ELECTRON FLOW ; PHOTOSYSTEM-I ; MEMBRANE ; GENES ; NADPH ; G6PDH ; NACL
资助项目Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2022LZGC004] ; National Natural Science Foundation of China[42376091] ; National Natural Science Foundation of China[41606159] ; Foundation of State Key Laboratory of Mariculture Biobreeding and Sustainable Goods[BRESG202308] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; Agriculture Research System of China of MOF and MARA[CARS-50]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:001240029000001
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/186035]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Wang, Guangce
作者单位1.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
2.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
4.Qingdao Agr Univ, Sci Life Coll, Qingdao, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Shi, Mengmeng,Lin, Guohong,Zhang, Baoyu,et al. Expression characteristics and interference analysis of glucose-6-phosphate dehydrogenase reveal its importance in Pyropia yezoensis under salt stress[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2024,80:7.
APA Shi, Mengmeng.,Lin, Guohong.,Zhang, Baoyu.,Xie, Xiujun.,Qiang, Xi.,...&Wang, Guangce.(2024).Expression characteristics and interference analysis of glucose-6-phosphate dehydrogenase reveal its importance in Pyropia yezoensis under salt stress.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,80,7.
MLA Shi, Mengmeng,et al."Expression characteristics and interference analysis of glucose-6-phosphate dehydrogenase reveal its importance in Pyropia yezoensis under salt stress".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 80(2024):7.

入库方式： OAI收割

来源：海洋研究所

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