Impact of O-acetylation on chitin oligosaccharides modulating inflammatory responses in LPS-induced RAW264.7 cells and mice
文献类型：期刊论文
| 作者 | Ge, Xiangyun1,3; Zhu, Siqi3; Yang, Haoyue3 ; Wang, Xin1,3; Li, Jingwen3; Liu, Song2,3; Xing, Ronge2,3; Li, Pengcheng2,3; Li, Kecheng2,3
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| 刊名 | CARBOHYDRATE RESEARCH
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| 出版日期 | 2024-08-01 |
| 卷号 | 542页码:11 |
| 关键词 | Chitin oligosaccharides O-acetylation Immunomodulatory activity Endotoxemia |
| ISSN号 | 0008-6215 |
| DOI | 10.1016/j.carres.2024.109177 |
| 通讯作者 | Li, Kecheng(lkc@qdio.ac.cn) |
| 英文摘要 | Chitin oligosaccharides have garnered significant attention due to their biological activities, particularly their immunomodulatory properties. However, O-acetylation in chemically preparing chitin oligosaccharides seems inevitable and leads to some uncertainty on the bioactivity of chitin oligosaccharides. In this study, an O-acetylfree chitin oligosaccharides and three different O-acetylated chitin oligosaccharides with degree of polymerization ranging from 2 to 6 were prepared using ammonia hydrolysis, and their structures and detailed components were further characterized with FTIR, NMR and MS. Subsequently, the effects of O-acetylation on the immunomodulatory activity of chitin oligosaccharides were investigated in vitro and in vivo. The results suggested that the chitin oligosaccharides with O-acetylation exhibited better inflammatory inhibition than pure chitin oligosaccharides, significantly reducing the expression of inflammatory factors, such as IL-6 and iNOS, in the LPS-induced RAW264.7 macrophage. The chitin oligosaccharides with a degree of O-acetylation of 93 % was found to effectively alleviate LPS-induced endotoxemia in mice, including serum inflammation indices reduction and damage repairment of the intestinal liver, and kidney tissues. |
| WOS关键词 | CHITOSAN OLIGOSACCHARIDES ; OXIDATIVE STRESS ; MOLECULAR-WEIGHT ; DEACETYLATION ; GLUCOMANNAN ; POLYSACCHARIDE ; MACROPHAGES ; BIOACTIVITY ; PI3K/AKT |
| 资助项目 | National Natural Science Foundation of China[42276097] ; Natural Science Foundation of Shandong Province[ZR2022YQ37] ; Shandong Key RD Plan ; Major Scientific and Technological Innovation Project[2022CXGC020413] ; Project for Future Network from Chinese Academy of Sciences[058GJHZ2023044FN] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001273117000001 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186081]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Kecheng |
| 作者单位 | 1.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Xiangyun,Zhu, Siqi,Yang, Haoyue,et al. Impact of O-acetylation on chitin oligosaccharides modulating inflammatory responses in LPS-induced RAW264.7 cells and mice[J]. CARBOHYDRATE RESEARCH,2024,542:11. |
| APA | Ge, Xiangyun.,Zhu, Siqi.,Yang, Haoyue.,Wang, Xin.,Li, Jingwen.,...&Li, Kecheng.(2024).Impact of O-acetylation on chitin oligosaccharides modulating inflammatory responses in LPS-induced RAW264.7 cells and mice.CARBOHYDRATE RESEARCH,542,11. |
| MLA | Ge, Xiangyun,et al."Impact of O-acetylation on chitin oligosaccharides modulating inflammatory responses in LPS-induced RAW264.7 cells and mice".CARBOHYDRATE RESEARCH 542(2024):11. |
入库方式： OAI收割
来源：海洋研究所
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