Green and eco-friendly method for the extraction of chitin from crab shells (Chionoecetes opilio) with Lactiplantibacillus plantarum and Pseudomonas aeruginosa
文献类型：期刊论文
| 作者 | Zhou, Yi2,3 ; Yang, Haoyue2; Li, Guantian2; Xing, Ronge1,2; Liu, Song1,2; Li, Kecheng1,2; Li, Rongfeng1,2; Yu, Huahua1,2
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| 刊名 | BIOMASS CONVERSION AND BIOREFINERY
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| 出版日期 | 2024-07-31 |
| 页码 | 16 |
| 关键词 | Chitin extraction Crab shells Microbiological method Antioxidant activity |
| ISSN号 | 2190-6815 |
| DOI | 10.1007/s13399-024-05970-y |
| 通讯作者 | Xing, Ronge(xingronge@qdio.ac.cn) |
| 英文摘要 | There are few reports on the extraction of chitin from crab shells using microbiological methods. In this work, the strain Lactiplantibacillus plantarum (LA01) for demineralization (DM) and the strain Pseudomonas aeruginosa (PS01) for deproteinization (DP) were screened from probiotic powders, soil, seawater, mud of aquaculture farm, seamount, and marine cold spring. After optimizing the fermentation conditions, PS01 followed by LA01 was chosen as the better inoculation sequence for two-step fermentation process to extract chitin from crab shells (Chionoecetes opilio). The chitin obtained by fermentation was analyzed by FTIR, XRD, SEM, and TGA. Results showed that 97.55 +/- 0.15% of calcium carbonate and 73.49 +/- 0.25% of protein were eliminated. The strong connection between protein and chitin in the crab shells was speculated as the reason of protein residues. A similar structure was observed in the crab chitin according to the results of structural analysis compared with commercial chitin, indicating the efficacy of microbiological methods used for chitin extraction from crab shells. The presence of porous and fibrous structure was observed in crab chitin according to the images of SEM. The organic acids in the supernatant of LA01 were mainly lactic acid (26,190.31 mg/L) and acetic acid (799.85 mg/L). The results of antioxidant activity assays showed that the fermentation supernatant possessed strong antioxidant activity, in which the scavenging activity of DPPH free radicals (96.50 +/- 1.68%) in LA01 was higher than that of 1.5 g/L ascorbic acid solution (96.18 +/- 0.43%), with 0.24 +/- 0.00 mg/mL of total phenolic and 7.54 +/- 0.31 mg/mL of exopolysaccharide. |
| WOS关键词 | MOLECULAR-WEIGHT CHITIN ; CHITOSAN ; SHRIMP ; WASTE ; FERMENTATION ; PRODUCTS |
| 资助项目 | The Key R&D Program (Major Scientific and Technological Innovation Project) of Shandong Province, China |
| WOS研究方向 | Energy & Fuels ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:001285090800004 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186198]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Xing, Ronge |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Yi,Yang, Haoyue,Li, Guantian,et al. Green and eco-friendly method for the extraction of chitin from crab shells (Chionoecetes opilio) with Lactiplantibacillus plantarum and Pseudomonas aeruginosa[J]. BIOMASS CONVERSION AND BIOREFINERY,2024:16. |
| APA | Zhou, Yi.,Yang, Haoyue.,Li, Guantian.,Xing, Ronge.,Liu, Song.,...&Yu, Huahua.(2024).Green and eco-friendly method for the extraction of chitin from crab shells (Chionoecetes opilio) with Lactiplantibacillus plantarum and Pseudomonas aeruginosa.BIOMASS CONVERSION AND BIOREFINERY,16. |
| MLA | Zhou, Yi,et al."Green and eco-friendly method for the extraction of chitin from crab shells (Chionoecetes opilio) with Lactiplantibacillus plantarum and Pseudomonas aeruginosa".BIOMASS CONVERSION AND BIOREFINERY (2024):16. |
入库方式： OAI收割
来源：海洋研究所
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