The small GTPase Cdc42 regulates shell field morphogenesis in a gastropod mollusk
文献类型：期刊论文
| 作者 | Liu, Xinyu1,4; Huan, Pin1,2,3,4 ; Liu, Baozhong2,4
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| 刊名 | DEVELOPMENTAL BIOLOGY
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| 出版日期 | 2024-11-01 |
| 卷号 | 515页码:7-17 |
| 关键词 | Lottia peitaihoensis Shell development Cdc42 F-actin Shell field morphogenesis |
| ISSN号 | 0012-1606 |
| DOI | 10.1016/j.ydbio.2024.06.012 |
| 通讯作者 | Huan, Pin(huanpin@qdio.ac.cn) |
| 英文摘要 | In most mollusks (conchiferans), the early tissue responsible for shell development, namely, the shell field, shows a common process of invagination during morphogenesis. Moreover, lines of evidence indicated that shell field invagination is not an independent event, but an integrated output reflecting the overall state of shell field morphogenesis. Nevertheless, the underlying mechanisms of this conserved process remain largely unknown. We previously found that actomyosin networks (regularly organized filamentous actin (F-actin) and myosin) may play essential roles in this process by revealing the evident aggregation of F-actin in the invaginated region and demonstrating that nonmuscle myosin II (NM II) is required for invagination in the gastropod Lottia peitaihoensis ( = Lottia goshimai ). Here, we investigated the roles of the Rho family of small GTPases (RhoA, Rac1, and Cdc42) to explore the upstream regulators of actomyosin networks. Functional assays using small molecule inhibitors suggested that Cdc42 modulates key events of shell field morphogenesis, including invagination and cell rearrangements, while the roles of RhoA and Rac1 may be nonspecific or negligible. Further investigations revealed that the Cdc42 protein was concentrated on the apical side of shell field cells and colocalized with F-actin aggregation. The aggregation of these two molecules could be prevented by treatment with Cdc42 inhibitors. These findings suggest a possible regulatory cascade of shell field morphogenesis in which Cdc42 recruits F-actin (actomyosin networks) on the apical side of shell field cells, which then generates resultant mechanical forces that mediate correct shell field morphogenesis (cell shape changes, invagination and cell rearrangement). Our results emphasize the roles of the cytoskeleton in early shell development and provide new insights into molluscan shell evolution. |
| WOS关键词 | EMBRYONIC-DEVELOPMENT ; RHO-GTPASES ; CO-OPTION ; PERIOSTRACUM ; EXPRESSION ; ARCHITECTURE ; VENERIDAE ; FILOPODIA ; EVOLUTION ; PATHWAY |
| 资助项目 | Science & Technology Innovation Project of Laoshan Laboratory[LSKJ202203002] ; National Natural Science Foundation of China[41776157] ; National Natural Science Foundation of China[42076123] ; Earmarked fund for CARS-49 ; Taishan Scholar Project Fund of Shandong Province of China[tsqn202103129] |
| WOS研究方向 | Developmental Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001266276600001 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186311]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Huan, Pin |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xinyu,Huan, Pin,Liu, Baozhong. The small GTPase Cdc42 regulates shell field morphogenesis in a gastropod mollusk[J]. DEVELOPMENTAL BIOLOGY,2024,515:7-17. |
| APA | Liu, Xinyu,Huan, Pin,&Liu, Baozhong.(2024).The small GTPase Cdc42 regulates shell field morphogenesis in a gastropod mollusk.DEVELOPMENTAL BIOLOGY,515,7-17. |
| MLA | Liu, Xinyu,et al."The small GTPase Cdc42 regulates shell field morphogenesis in a gastropod mollusk".DEVELOPMENTAL BIOLOGY 515(2024):7-17. |
入库方式： OAI收割
来源：海洋研究所
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