Molecular Characterization of the Actin Gene and 5′ Flanking Sequence from Brown Macroalga Saccharina japonica (Laminariales, Phaeophyta)
文献类型：期刊论文
| 作者 | Xu, Hao1,2,3,4; Wang, Zhenghua1,2,3,4; Zhang, Yichen2,3,5; Jiang, Peng2,3,4 |
| 刊名 | JOURNAL OF MARINE SCIENCE AND ENGINEERING
 |
| 出版日期 | 2024-06-01 |
| 卷号 | 12期号:6页码:13 |
| 关键词 | actin Biolistic GFP lacZ constitutive promoter Saccharina japonica transient expression |
| DOI | 10.3390/jmse12060887 |
| 通讯作者 | Jiang, Peng(jiangpeng@qdio.ac.cn) |
| 英文摘要 | The brown macroalga Saccharina japonica (Laminariales, Phaeophyta) is the most productive cultured seaweed in the world. In order to improve the biosafety of transgenic kelp, it is necessary to develop endogenous constitutive promoters, replacing those of virus origin. In this study, the housekeeping actin gene from S. japonica (SjACT) was found to contain three exons and two introns, representing a unique actin gene structure pattern in brown algae. Additionally, the 5 ' upstream region was obtained using genome walking, and fused to the reporter gene lacZ or EGFP to construct promoter-detective vectors. Using an established genetic transformation system, kelps in different life-cycle stages were transformed. The detection results showed that, in the diploid sporophyte stage, the transient expression from the lacZ gene could be observed in the frond, stipe, or holdfast of kelps, indicating a manner of being non-tissue-specific. And, in the haploid gametophyte stage of S. japonica, the fluorescence of the expressed EGFP were detected in vivo in gametophyte cells of both genders. These results indicate that the promoter of the SjACT gene (pSjACT) functions in a constitutive manner and is expected to be a key endogenous element in the genetic manipulation of kelps. |
| WOS关键词 | STRUCTURAL FEATURES ; EXPRESSION ; PHYLOGENY ; PROMOTER ; ALGAE |
| 资助项目 | Natural Science Foundation of Qingdao City[23-2-1-172-zyyd-jch] ; Science & Technology Basic Resources Investigation Program of China[2018FY100205] ; Key R&D Program of Shandong Province[2019GSF107012] ; National Key R&D Program of China[2018YFD0901500] ; Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province[2010BSB02009] |
| WOS研究方向 | Engineering ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:001257569700001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186380]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Jiang, Peng |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 5.Tianjin Normal Univ, Coll Life Sci, Tianjin 300387, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Hao,Wang, Zhenghua,Zhang, Yichen,et al. Molecular Characterization of the Actin Gene and 5′ Flanking Sequence from Brown Macroalga Saccharina japonica (Laminariales, Phaeophyta)[J]. JOURNAL OF MARINE SCIENCE AND ENGINEERING,2024,12(6):13. |
| APA | Xu, Hao,Wang, Zhenghua,Zhang, Yichen,&Jiang, Peng.(2024).Molecular Characterization of the Actin Gene and 5′ Flanking Sequence from Brown Macroalga Saccharina japonica (Laminariales, Phaeophyta).JOURNAL OF MARINE SCIENCE AND ENGINEERING,12(6),13. |
| MLA | Xu, Hao,et al."Molecular Characterization of the Actin Gene and 5′ Flanking Sequence from Brown Macroalga Saccharina japonica (Laminariales, Phaeophyta)".JOURNAL OF MARINE SCIENCE AND ENGINEERING 12.6(2024):13. |
入库方式： OAI收割
来源：海洋研究所
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