CircRNA8388 functions as the sponge for miR-2392 during intestinal regeneration in sea cucumber Apostichopus japonicus
文献类型：期刊论文
| 作者 | Su, Fang ; Huo, Da; Yang, Hongsheng; Sun, Lina2
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| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2024-08-01 |
| 卷号 | 274页码:7 |
| 关键词 | Circular RNA Intestinal regeneration Apostichopus japonicus |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2024.133302 |
| 通讯作者 | Sun, Lina(sunlina@qdio.ac.cn) |
| 英文摘要 | The sea cucumber Apostichopus japonicus can expel internal organs under stress and regenerate them subsequently. However, growth is delayed during regeneration, significantly impacting the industry. Circular RNAs (circRNAs) are single-stranded circular RNA molecules produced through alternative splicing of mRNA precursors. They play crucial roles in regulating gene expression via the ceRNA mechanism. In this study, circRNA profiles of control and regenerated intestines were constructed. A total of 15,874 circRNAs were identified, with a length of 300-350 nucleotides (nt) being the most abundant. Sanger sequencing confirmed the circular structure of circRNA398. Compared with the normal intestine, 50 and 83 differentially expressed circRNAs (DEcircRNAs) were identified in the regenerated intestine at 1 and 3 days post evisceration (dpe), respectively. Gene ontology (GO) terms for signal transduction and development regulation were most significantly enriched in 1dpeVScon and 3dpeVScon treatments, respectively. The dual-luciferase assay revealed that circRNA8388 functions as a sponge for miR-2392, participating in the remodeling of the extracellular matrix (ECM). In conclusion, these findings will contribute to the enhancement of the non-coding RNA database for echinoderms and lay the groundwork for future investigations into circRNA regulation during intestinal regeneration. |
| WOS关键词 | CIRCULAR RNAS |
| 资助项目 | Foundation of Shandong Province[202306279] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001262117300001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186395]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Lina |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Fang,Huo, Da,Yang, Hongsheng,et al. CircRNA8388 functions as the sponge for miR-2392 during intestinal regeneration in sea cucumber Apostichopus japonicus[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,274:7. |
| APA | Su, Fang,Huo, Da,Yang, Hongsheng,&Sun, Lina.(2024).CircRNA8388 functions as the sponge for miR-2392 during intestinal regeneration in sea cucumber Apostichopus japonicus.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,274,7. |
| MLA | Su, Fang,et al."CircRNA8388 functions as the sponge for miR-2392 during intestinal regeneration in sea cucumber Apostichopus japonicus".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 274(2024):7. |
入库方式： OAI收割
来源：海洋研究所
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