Pufferfish gasdermin Ea is a significant player in the defense against bacterial pathogens
文献类型：期刊论文
| 作者 | Xu, Hang1,2,3,4 ; Qin, Kunpeng1,2,3,4; Hao, Kangwei1,2,3,4; Yuan, Zihao1,2,3,4; Sun, Li1,2,3,4
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| 刊名 | MARINE LIFE SCIENCE & TECHNOLOGY
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| 出版日期 | 2024-06-28 |
| 页码 | 13 |
| 关键词 | GSDME Caspase Takifugu rubripes Bacterial infection Immune defense |
| ISSN号 | 2096-6490 |
| DOI | 10.1007/s42995-024-00237-x |
| 通讯作者 | Yuan, Zihao(yuanzihao@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | Gasdermins (GSDMs) are proteins cleaved by caspase (CASP) to trigger pyroptosis. In teleosts, pyroptosis is mediated by gasdermin E (GSDME). The Pufferfish, Takifugu rubripes, possesses two GSDME orthologs: named TrGSDMEa and TrGSDMEb. TrGSDMEa is cleaved by CASP3/7 to liberate the N-terminal (NT) domain that can trigger pyroptosis in mammalian cells. However, the biological function of TrGSDMEa in pufferfish is unknown, and TrGSDMEb is poorly studied. We found that TrGSDMEb was cleaved by CASP1/3/6/7/8, but the resulting NT domain, despite its similarity to TrGSDMEa-NT domain in sequence and structure, failed to induce pyroptosis. TrGSDMEa and TrGSDMEb exhibited similar expression patterns in pufferfish under normal physiological conditions but were up- and downregulated, respectively, in expression during Vibrio harveyi and Edwardsiella tarda infection. Bacterial infection induced the activation of TrGSDMEa and CASP3/7 in pufferfish cells, resulting in pyroptosis accompanied with IL-1 beta production and maturation. Inhibition of TrGSDMEa-mediated pyroptosis via TrCASP3/7 reduced the death of pufferfish cells and augmented bacterial dissemination in fish tissues. Structure-oriented mutagenesis identified 16 conserved residues in teleost GSDMEa that were required for the pore formation or auto-inhibition of GSDMEa. This study illustrates the role of GSDMEa-mediated pyroptosis in teleost defense against bacterial pathogens and provides new insights into the structure-based function of vertebrate GSDME. |
| WOS关键词 | INFLAMMATORY CASPASES ; PYROPTOSIS ; CLEAVAGE ; ACTIVATION ; GENOME ; FAMILY ; PORE |
| 资助项目 | Science & Technology Innovation Project of Laoshan Laboratory[LSKJ202203000] ; Postdoctoral Fellowship Program of CPSF[GZC20232709] ; China Postdoctoral Science Foundation[2023TQ0356] |
| WOS研究方向 | Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001258613400001 |
| 出版者 | SPRINGERNATURE |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186407]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Yuan, Zihao; Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Laoshan Lab, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Sch Marine Sci, Qingdao 266400, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Hang,Qin, Kunpeng,Hao, Kangwei,et al. Pufferfish gasdermin Ea is a significant player in the defense against bacterial pathogens[J]. MARINE LIFE SCIENCE & TECHNOLOGY,2024:13. |
| APA | Xu, Hang,Qin, Kunpeng,Hao, Kangwei,Yuan, Zihao,&Sun, Li.(2024).Pufferfish gasdermin Ea is a significant player in the defense against bacterial pathogens.MARINE LIFE SCIENCE & TECHNOLOGY,13. |
| MLA | Xu, Hang,et al."Pufferfish gasdermin Ea is a significant player in the defense against bacterial pathogens".MARINE LIFE SCIENCE & TECHNOLOGY (2024):13. |
入库方式： OAI收割
来源：海洋研究所
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