Exploration and origin studies of high levels of β-glucosidase in carnivorous fishes spotted knifejaw ( Oplegnathus punctatus )
文献类型：期刊论文
| 作者 | Wu, Yanduo2,3,6; Xiao, Yongshuang1,2,3; Xiao, Zhizhong1,2,3,4; Li, Wensheng5; Li, Jun1,2,3 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2024-07-01 |
| 卷号 | 273页码:14 |
| 关键词 | Oplegnathus punctatus Dietary habits beta-Glucosidase Cellulose Intestinal microorganisms |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2024.132929 |
| 通讯作者 | Xiao, Yongshuang(yongshuangxiao@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| 英文摘要 | In order to more efficiently utilize the abundant cellulose resources in nature, increase the utilization rate of cellulose in aquaculture, implement precise feeding and save aquaculture costs, we have conducted research on cellulase genes related to the spotted knifejaw ( Oplegnathus punctatus ). Cellulose, as the most abundant renewable resource, is a cornerstone in the intricate ecological balance of diverse ecosystems. While herbivorous fish are recognized for their utilization of proteins, sugars, and fats, the extent of cellulose utilization by carnivorous and omnivorous fish remains an enigma. Here, through field sampling and behavioural observations, O. punctatus ' omnivorous diet has been demonstrated (stomach contents contain approximately several species of algae in the Bacillariophyta (1.12 %), Streptomyces (0.55 %), Chlorophyta (0.35 %), Rhodophyta (0.16 %), and Euglenophyta (0.19 %) phylum). Additionally, the high cellulase activity in the intestine of O. punctatus has been detected first discovery (enzyme activity up to 4800.15 U/g), indicating its ability to digest cellulose. By employing whole-genome scanning and high-throughput sequencing, a single cellulase gene ( beta -glucosidase ) within the genome of O. punctatus , suggesting the absence of a complete cellulose digestive system. However, microbiological analysis revealed the three crucial role of microorganisms, including Actinobacteria (25.80 %), Bacteroidetes (18.93 %), and Firmicutes phylum (0.82 %), were found to play a crucial role in the decomposition of plant cell walls, thereby facilitating plant material digestion to help the host to complete the process of cellulose digestion. Expression patterns and proteomic analysis of the beta -glucosidase were notably high in the gonads. In situ hybridization confirmed the expression of the beta -glucosidase gene in the intestinal contents and gonads, highlighting its role in supplying energy of gonads. These discoveries shed light on the dietary habits of O. punctatus and its cellulose utilization, offering insights that can inform the development of customized feeding strategies to enhance aquaculture sustainability and minimize resource expenditure. |
| WOS关键词 | ENZYMATIC-HYDROLYSIS ; MOLECULAR-CLONING ; CELLULASE GENE |
| 资助项目 | National Natural Science Foundation of China[42276107] ; National Key Research and Development Program[2022YFC3103600] ; Shandong Province Key R D Program[2023LZGCQY001] ; Key Deployment Projects of Center for Ocean Mega-Science, Chinese Academy of Sciences[COMS2020Q05] ; Key Deployment Projects of Center for Ocean Mega-Science, Chinese Academy of Sciences[COMS2020Q05] ; [CARS-47] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001255218600001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186428]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Xiao, Yongshuang; Li, Jun |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Weihai Hao Huigan Marine Biotechnol Co, Weihai, Peoples R China 5.Laizhou Mingbo Aquat Prod Co Ltd, Yantai, Peoples R China 6.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Yanduo,Xiao, Yongshuang,Xiao, Zhizhong,et al. Exploration and origin studies of high levels of β-glucosidase in carnivorous fishes spotted knifejaw ( Oplegnathus punctatus )[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,273:14. |
| APA | Wu, Yanduo,Xiao, Yongshuang,Xiao, Zhizhong,Li, Wensheng,&Li, Jun.(2024).Exploration and origin studies of high levels of β-glucosidase in carnivorous fishes spotted knifejaw ( Oplegnathus punctatus ).INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,273,14. |
| MLA | Wu, Yanduo,et al."Exploration and origin studies of high levels of β-glucosidase in carnivorous fishes spotted knifejaw ( Oplegnathus punctatus )".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 273(2024):14. |
入库方式： OAI收割
来源：海洋研究所
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