Overexpression of MPV17/PMP22-like protein 2 gene decreases production of radical oxygen species in Pyropia yezoensis (Bangiales, Rhodophyta)
文献类型：期刊论文
| 作者 | Li, Yujie1,2; Yang, Jiali1,2 ; Sun, Zhenjie2; Niu, Jianfeng2; Wang, Guangce2,3,4
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| 刊名 | JOURNAL OF PHYCOLOGY
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| 出版日期 | 2024-06-24 |
| 页码 | 14 |
| 关键词 | antioxidant capacity genetic breeding peroxisomal porin-like channel photorespiratory photosynthetic electron chain Pyropia yezoensis |
| ISSN号 | 0022-3646 |
| DOI | 10.1111/jpy.13474 |
| 通讯作者 | Niu, Jianfeng(jf_niu@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn) |
| 英文摘要 | The northward shift of Pyropia yezoensis aquaculture required the breeding of germplasms with tolerance to the oxidative stress due to the high light conditions of the North Yellow Sea area. The MPV17/PMP22 family proteins were identified as a molecule related to reactive oxygen species (ROS) metabolism. Here, one of the MPV17 homolog genes designated as PyM-LP2 was selected for functional identification by introducing the encoding sequence region/reverse complementary fragment into the Py. yezoensis genome. Although the photosynthetic activity, the respiratory rate, and the ROS level in wild type (WT) and different gene-transformed algal strains showed similar levels under normal conditions, the overexpression (OE) strain exhibited higher values of photosynthesis, respiration, and reducing equivalents pool size but lower intracellular ROS production under stress conditions compared with the WT. Conversely, all the above parameters showed opposite variation trends in RNAi strain as those in the OE strain. This implied that the PyM-LP2 protein was involved in the mitigation of the oxidative stress. Sequence analysis revealed that this PyM-LP2 protein was assorted to peroxisomes and might serve as a poring channel for transferring malate (Mal) to peroxisomes. By overexpressing PyM-LP2, the transfer of Mal from chloroplasts to peroxisomes was enhanced under stress conditions, which promoted photorespiration and ultimately alleviated excessive reduction of the photosynthetic electron chain. This research lays the groundwork for the breeding of algae with enhanced resistance to oxidative stresses. |
| WOS关键词 | PEROXISOMAL TARGETING SIGNAL ; SACCHAROMYCES-CEREVISIAE ; EXPRESSION ; MEMBRANE ; PHOTOSYNTHESIS ; CHLOROPLASTS ; RESPIRATION ; PROMOTER ; RECEPTOR ; STRESS |
| 资助项目 | Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2022LZGC004] ; National Natural Science Foundation of China[42376091] ; China Agriculture Research System of MOF and MARA[CARS-50] ; Foundation of State Key Laboratory of Mariculture Biobreeding and Sustainable Goods[BRESG202308] ; Opening Foundation of Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, CAS[2023FB12] ; Research Fund for the Taishan Scholar Project of Shandong Province[2023FB12] |
| WOS研究方向 | Plant Sciences ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001253940800001 |
| 出版者 | WILEY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186437]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Niu, Jianfeng; Wang, Guangce |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yujie,Yang, Jiali,Sun, Zhenjie,et al. Overexpression of MPV17/PMP22-like protein 2 gene decreases production of radical oxygen species in Pyropia yezoensis (Bangiales, Rhodophyta)[J]. JOURNAL OF PHYCOLOGY,2024:14. |
| APA | Li, Yujie,Yang, Jiali,Sun, Zhenjie,Niu, Jianfeng,&Wang, Guangce.(2024).Overexpression of MPV17/PMP22-like protein 2 gene decreases production of radical oxygen species in Pyropia yezoensis (Bangiales, Rhodophyta).JOURNAL OF PHYCOLOGY,14. |
| MLA | Li, Yujie,et al."Overexpression of MPV17/PMP22-like protein 2 gene decreases production of radical oxygen species in Pyropia yezoensis (Bangiales, Rhodophyta)".JOURNAL OF PHYCOLOGY (2024):14. |
入库方式： OAI收割
来源：海洋研究所
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