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In situ real-time pathway to study the polyethylene long-term degradation process by a marine fungus through confocal Raman quantitative imaging

文献类型：期刊论文

作者He, Wanying2,3,4; Liu, Rui1,4,5,6; Fei, Fan1,4,5,6; Xi, Shichuan2,3,4; Du, Zengfeng2,3,4; Luan, Zhendong1,2,3,4; Sun, Chaomin1,2,4,5,6,7; Zhang, Xin1,2,3,4,7
刊名SCIENCE OF THE TOTAL ENVIRONMENT
出版日期2024-08-20
卷号939页码:7
关键词Marine fungus Polyethylene biodegradation Confocal Raman microscopy In situ Real-time
ISSN号0048-9697
DOI10.1016/j.scitotenv.2024.173582
通讯作者Sun, Chaomin(sunchaomin@qdio.ac.cn) ; Zhang, Xin(xzhang@qdio.ac.cn)
英文摘要Since plastic waste has become a worldwide pollution problem, studying the ability of marine microorganisms to degrade plastic waste is important. However, conventional methods are unable to in situ real-time study the ability of microorganisms to biodegrade plastics. In recent years, Raman spectroscopy has been widely used in the characterization of plastics as well as in the study of biological metabolism due to its low cost, rapidity, labelfree, non-destructive, and water -independent features, which provides us with new ideas to address the above limitations. Here, we have established a method to study the degradation ability of microorganisms on plastics using confocal Raman imaging. Alternaria alternata FB1, a recently reported polyethylene (PE) degrading marine fungus, is used as a model to perform a long-term (up to 274 days) in situ real-time nondestructive inspection of its degradation process. We can prove the degradation of PE plastics from the following two aspects, visualization and analysis of the degradation process based on depth imaging and quantification of the degradation rate by crystallinity calculations. The findings also reveal unprecedented degradation details. The method is important for realizing high-throughput screening of microorganisms with potential to degrade plastics and studying the degradation process of plastics in the future.
WOS关键词CRYSTALLINITY ; SPECTROSCOPY
资助项目National Natural Science Foundation of China[42221005] ; National Natural Science Foundation of China[42327805] ; Key technology research project of cold spring equipment, Chinese Academy of Sciences[LQ-GJ-03-02] ; Key project of Ocean Research Center, Chinese Academy of Sciences[COMS2020J03] ; Key project of Ocean Research Center, Chinese Academy of Sciences[COMS2020Q04] ; Young Taishan Scholars Program[tsqn201909158] ; Science and Technology Innovation Project of Laoshan Laboratory[LSKJ202203103] ; Shandong Provincial Natural Science Foundation[ZR2021ZD28] ; Taishan Scholars Program[tstp20230637] ; Key Collaborative Research Program of the Alliance of International Science Organizations[ANSO-CR-KP-2022-08] ; Natural Science Foundation of Qingdao[23-2-1-182-zyyd-jch]
WOS研究方向Environmental Sciences & Ecology
语种英语
WOS记录号WOS:001250773500001
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/186488]  
专题海洋研究所_海洋地质与环境重点实验室
海洋研究所_实验海洋生物学重点实验室
通讯作者Sun, Chaomin; Zhang, Xin
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Laoshan Lab, Qingdao, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Geol & Environm, Qingdao, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
6.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
7.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 He, Wanying,Liu, Rui,Fei, Fan,et al. In situ real-time pathway to study the polyethylene long-term degradation process by a marine fungus through confocal Raman quantitative imaging[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2024,939:7.
APA He, Wanying.,Liu, Rui.,Fei, Fan.,Xi, Shichuan.,Du, Zengfeng.,...&Zhang, Xin.(2024).In situ real-time pathway to study the polyethylene long-term degradation process by a marine fungus through confocal Raman quantitative imaging.SCIENCE OF THE TOTAL ENVIRONMENT,939,7.
MLA He, Wanying,et al."In situ real-time pathway to study the polyethylene long-term degradation process by a marine fungus through confocal Raman quantitative imaging".SCIENCE OF THE TOTAL ENVIRONMENT 939(2024):7.

入库方式： OAI收割

来源：海洋研究所

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