Correlation of glutamate dehydrogenase gene polymorphism and mRNA expression level with free glutamate content in the clam Meretrix petechialis
文献类型：期刊论文
| 作者 | Tian, Yanmin3,4; Liu, Baozhong1,2,4 ; Yue, Xin4; Wang, Hongxia4
|
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2024-10-15 |
| 卷号 | 591页码:9 |
| 关键词 | Meretrix petechialis Glutamate dehydrogenase Free glutamate content Gene polymorphism SNP |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2024.741130 |
| 通讯作者 | Wang, Hongxia(hxwang@qdio.ac.cn) |
| 英文摘要 | Free glutamate is a characteristic amino acid with a fresh taste. A high free glutamate content can increase the flavour of clams and better meet consumer demands. GDH is involved in the production of glutamate from a-ketoglutarate and determines the speed and direction of reaction in the glutamate metabolic pathway. In this study, we cloned a full-length cDNA of MpGDH homologues in the clam Meretrix petechialis and further investigated the relationship among MpGDH gene polymorphisms, gene expression levels, enzyme activity and glutamate content traits. A significant positive correlation of MpGDH gene expression with free glutamate content was observed (r = 0.62, p < 0.05). The relationship of variation in MpGDH gene expression with its SNP genotype and SNP haplotype was assessed. Five SNPs were found to be significantly associated with MpGDH gene expression (p < 0.05), from which we constructed two main haplotypes due to linkage disequilibrium. The results showed that haplotype H2 may be an effective haplotype associated with high MpGDH gene expression and high free glutamate content. The genetic effect of different haplotypes was validated by different family materials, which showed that MpGDH gene expression level of the H1H2 heterozygous genotype were significantly higher than those of the homozygous genotype H1H1 (p < 0.05). In summary, our findings revealed the potential influence of the MpGDH polymorphism on the free glutamate content and provided molecular biological information for the selective breeding of good-quality traits of M. petechialis. |
| WOS关键词 | MOLECULAR CHARACTERIZATION ; VIBRIO-RESISTANCE ; METABOLISM ; CHALLENGE ; GROWTH ; GDH |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030304] ; Key Research and Development Program of Shandong ; Taishan Industrial Leading Talents Project to Shandong Dehe Mingxing Biotechnology Co., Ltd. ; Natural Science Foundation of Shandong Province[ZR2020MD089] ; [CARS-49] ; [2021LZGC029] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001247977900002 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186572]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Hongxia |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 2.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Yanmin,Liu, Baozhong,Yue, Xin,et al. Correlation of glutamate dehydrogenase gene polymorphism and mRNA expression level with free glutamate content in the clam Meretrix petechialis[J]. AQUACULTURE,2024,591:9. |
| APA | Tian, Yanmin,Liu, Baozhong,Yue, Xin,&Wang, Hongxia.(2024).Correlation of glutamate dehydrogenase gene polymorphism and mRNA expression level with free glutamate content in the clam Meretrix petechialis.AQUACULTURE,591,9. |
| MLA | Tian, Yanmin,et al."Correlation of glutamate dehydrogenase gene polymorphism and mRNA expression level with free glutamate content in the clam Meretrix petechialis".AQUACULTURE 591(2024):9. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。