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Dinoflagellate Tripos species composition and seasonal dynamics in Jiaozhou Bay revealed through 18S rDNA V4-based metabarcoding analysis

文献类型：期刊论文

作者Huang, Xianliang3,4,5,6; Liu, Kuiyan3,4,5,6; Ding, Xiangxiang3,4,5,6; Liu, Shuya3,5,6; Cui, Zhongmei3,5,6; Zhao, Yongfang1,5,6; Chen, Nansheng2,3,5,6
刊名JOURNAL OF APPLIED PHYCOLOGY
出版日期2024-04-15
页码14
关键词Tripos Harmful algal bloom Composition Spatiotemporal Molecular marker Metabarcoding
ISSN号0921-8971
DOI10.1007/s10811-024-03239-5
通讯作者Chen, Nansheng(chenn@qdio.ac.cn)
英文摘要Tripos is one of the largest dinoflagellate genera with similar to 800 reported taxa widely distributed in global marine ecosystems. These diverse Tripos species are ecologically important as critical primary producers and some Tripos species are also harmful algal bloom (HAB) species that may pose significant negative impact on marine ecosystems. However, due to the high morphological intraspecific variability and plasticity of numerous Tripos species, morphology-based identification and tracking of their dynamic changes have been both technically challenging and inconclusive. In this project, we carried out metabarcoding analysis of Tripos species in Jiaozhou Bay (JZB) through amplifying and sequencing of the common molecular marker 18S rDNA V4. The analysis identified five Tripos species (T. furca, T. fusus, T. eugrammus, T. falcatus and T. massiliensis), including two (T. eugrammus and T. falcatus) identified for the first time in JZB, demonstrating the competitive strength of metabarcoding analysis. This analysis of time-series samples collected in JZB revealed interesting seasonal dynamic changes of Tripos species, with different species demonstrating differential seasonal preferences. This analysis also suggested the possible existence of 11 additional Tripos species in JZB. The inconclusive identification of these 11 potential Tripos species indicated that the resolution of the common molecular marker 18S rDNA V4 applied in this study is inadequate and that the molecular reference data for Tripos is still limited. Selection of molecular markers with higher resolution (such as 28S rDNA D1-6) and the completion of reference sequences of Tripos species may facilitate more accurate identification of and tracking of their dynamic changes.
WOS关键词HARMFUL ALGAL BLOOMS ; RED-TIDE ; GENUS CERATIUM ; SAGAMI BAY ; PHYTOPLANKTON ; BIODIVERSITY ; FURCA ; DINOPHYCEAE ; MIXOTROPHY ; POPULATION
资助项目Natural Science Foundation of China[42176162] ; National Key Research and Development (R&D) Program of China[2022YFC3105200] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[LSKJ202203700] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000] ; Chinese Academy of Sciences Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund
WOS研究方向Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种英语
WOS记录号WOS:001204763700001
出版者SPRINGER
源URL[http://ir.qdio.ac.cn/handle/337002/185531]  
专题海洋研究所_海洋生态与环境科学重点实验室
海洋研究所_胶州湾海洋生态系统国家野外研究站
通讯作者Chen, Nansheng
作者单位1.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Natl Marine Ecosyst Res Stn, Qingdao 266071, Peoples R China
2.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada
3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Coll Marine Sci, Beijing 100039, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China
推荐引用方式
GB/T 7714		 Huang, Xianliang,Liu, Kuiyan,Ding, Xiangxiang,et al. Dinoflagellate Tripos species composition and seasonal dynamics in Jiaozhou Bay revealed through 18S rDNA V4-based metabarcoding analysis[J]. JOURNAL OF APPLIED PHYCOLOGY,2024:14.
APA Huang, Xianliang.,Liu, Kuiyan.,Ding, Xiangxiang.,Liu, Shuya.,Cui, Zhongmei.,...&Chen, Nansheng.(2024).Dinoflagellate Tripos species composition and seasonal dynamics in Jiaozhou Bay revealed through 18S rDNA V4-based metabarcoding analysis.JOURNAL OF APPLIED PHYCOLOGY,14.
MLA Huang, Xianliang,et al."Dinoflagellate Tripos species composition and seasonal dynamics in Jiaozhou Bay revealed through 18S rDNA V4-based metabarcoding analysis".JOURNAL OF APPLIED PHYCOLOGY (2024):14.

入库方式： OAI收割

来源：海洋研究所

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