Extensive intragenomic variations of the 18S rDNA V4 region in the toxigenic diatom species Pseudo-nitzschia multistriata revealed through high-throughput sequencing
文献类型：期刊论文
| 作者 | Wang, Hui1,2,5,6 ; Liu, Kuiyan1,2,5,6; Chen, Ziyan1,2,5,6; Chen, Yang1,2,5,6; Hu, Zhangxi4; Chen, Weizhou8; Leaw, Chui Pin7; Chen, Nansheng1,2,3,5
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| 刊名 | MARINE POLLUTION BULLETIN
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| 出版日期 | 2024-04-01 |
| 卷号 | 201页码:11 |
| 关键词 | Metabarcoding analysis Pseudo-nitzschia multistriata Molecular markers polymorphism SSU rDNA Intragenomic variations (IGVs) |
| ISSN号 | 0025-326X |
| DOI | 10.1016/j.marpolbul.2024.116198 |
| 通讯作者 | Chen, Nansheng(chenn@qdio.ac.cn) |
| 英文摘要 | Metabarcoding analysis is an effective technique for monitoring the domoic acid-producing Pseudo-nitzschia species in marine environments, uncovering high-levels of molecular diversity. However, such efforts may result in the overinterpretation of Pseudo-nitzschia species diversity, as molecular diversity not only encompasses interspecies and intraspecies diversities but also exhibits extensive intragenomic variations (IGVs). In this study, we analyzed the V4 region of the 18S rDNA of 30 strains of Pseudo-nitzschia multistriata collected from the coasts of China. The results showed that each P. multistriata strain harbored about a hundred of unique 18S rDNA V4 sequence varieties, of which each represented by a unique amplicon sequence variant (ASV). This study demonstrated the extensive degree of IGVs in P. multistriata strains, suggesting that IGVs may also present in other Pseudo-nitzschia species and other phytoplankton species. Understanding the scope and levels of IGVs is crucial for accurately interpreting the results of metabarcoding analysis. |
| WOS关键词 | RIBOSOMAL-RNA GENES ; DOMOIC ACID ; PHYLOGENETIC-RELATIONSHIPS ; MARINE PROTISTS ; SP-NOV ; BACILLARIOPHYCEAE ; DIVERSITY ; DNA ; MORPHOLOGY ; TAXONOMY |
| 资助项目 | National Key Research and Development (R&D) Program of China[2022YFC3105200] ; Taishan Scholar Project Special Fund ; Natural Science Foundation of China[42176162] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000] ; Chinese Academy of Sciences Pioneer Hundred Talents Program |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001220642900001 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/185689]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Chen, Nansheng |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada 4.Guangdong Ocean Univ, Coll Fisheries, Dept Aquaculture, Zhanjiang 524088, Guangdong, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 6.Univ Chinese Acad Sci, Coll Marine Sci, Beijing 100039, Peoples R China 7.Univ Malaya, Inst Ocean & Earth Sci, Bachok Marine Res Stn, Bachok 16310, Kelantan, Malaysia 8.Shantou Univ, Inst Marine Biol, Shantou 515063, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hui,Liu, Kuiyan,Chen, Ziyan,et al. Extensive intragenomic variations of the 18S rDNA V4 region in the toxigenic diatom species Pseudo-nitzschia multistriata revealed through high-throughput sequencing[J]. MARINE POLLUTION BULLETIN,2024,201:11. |
| APA | Wang, Hui.,Liu, Kuiyan.,Chen, Ziyan.,Chen, Yang.,Hu, Zhangxi.,...&Chen, Nansheng.(2024).Extensive intragenomic variations of the 18S rDNA V4 region in the toxigenic diatom species Pseudo-nitzschia multistriata revealed through high-throughput sequencing.MARINE POLLUTION BULLETIN,201,11. |
| MLA | Wang, Hui,et al."Extensive intragenomic variations of the 18S rDNA V4 region in the toxigenic diatom species Pseudo-nitzschia multistriata revealed through high-throughput sequencing".MARINE POLLUTION BULLETIN 201(2024):11. |
入库方式： OAI收割
来源：海洋研究所
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