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Insights into phage-bacteria interaction in cold seep Gigantidas platifrons through metagenomics and transcriptome analyses

文献类型：期刊论文

作者Zhang, Yan2,6; Chen, Hao2,6; Lian, Chao2,6; Cao, Lei2,6; Guo, Yang2,6; Wang, Minxiao2,6; Zhong, Zhaoshan2,6; Li, Mengna1,2,6; Zhang, Huan1,2,6; Li, Chaolun2,3,4,5,6
刊名SCIENTIFIC REPORTS
出版日期2024-05-08
卷号14期号:1页码:12
关键词Viruses Gigantidas platifrons Metagenome Transcriptome Virus-host interaction
ISSN号2045-2322
DOI10.1038/s41598-024-61272-3
通讯作者Wang, Minxiao(wangminxiao@qdio.ac.cn) ; Li, Chaolun(lcl@qdio.ac.cn)
英文摘要Viruses are crucial for regulating deep-sea microbial communities and biogeochemical cycles. However, their roles are still less characterized in deep-sea holobionts. Bathymodioline mussels are endemic species inhabiting cold seeps and harboring endosymbionts in gill epithelial cells for nutrition. This study unveiled a diverse array of viruses in the gill tissues of Gigantidas platifrons mussels and analyzed the viral metagenome and transcriptome from the gill tissues of Gigantidas platifrons mussels collected from a cold seep in the South Sea. The mussel gills contained various viruses including Baculoviridae, Rountreeviridae, Myoviridae and Siphovirdae, but the active viromes were Myoviridae, Siphoviridae, and Podoviridae belonging to the order Caudovirales. The overall viral community structure showed significant variation among environments with different methane concentrations. Transcriptome analysis indicated high expression of viral structural genes, integrase, and restriction endonuclease genes in a high methane concentration environment, suggesting frequent virus infection and replication. Furthermore, two viruses (GP-phage-contig14 and GP-phage-contig72) interacted with Gigantidas platifrons methanotrophic gill symbionts (bathymodiolin mussels host intracellular methanotrophic Gammaproteobacteria in their gills), showing high expression levels, and have huge different expression in different methane concentrations. Additionally, single-stranded DNA viruses may play a potential auxiliary role in the virus-host interaction using indirect bioinformatics methods. Moreover, the Cro and DNA methylase genes had phylogenetic similarity between the virus and Gigantidas platifrons methanotrophic gill symbionts. This study also explored a variety of viruses in the gill tissues of Gigantidas platifrons and revealed that bacteria interacted with the viruses during the symbiosis with Gigantidas platifrons. This study provides fundamental insights into the interplay of microorganisms within Gigantidas platifrons mussels in deep sea.
WOS关键词DEEP-SEA SEDIMENTS ; COMMUNITIES ; OXIDATION ; ALIGNMENT ; HABITAT ; VIRUSES ; MUSSELS ; GENES ; OCEAN ; VENT
资助项目Laoshan Laboratory[2022QNLM030004] ; National Natural Science Foundation of China[42030407] ; National Natural Science Foundation of China[42106134] ; National Natural Science Foundation of China[42076091] ; National Natural Science Foundation of China[42221005] ; Key Research Program of Frontier Sciences[ZDBS-LY-DQC032] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050303] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42020401]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:001216508100056
出版者NATURE PORTFOLIO
源URL[http://ir.qdio.ac.cn/handle/337002/185880]  
专题海洋研究所_深海极端环境与生命过程研究中心
海洋研究所_海洋生态与环境科学重点实验室
通讯作者Wang, Minxiao; Li, Chaolun
作者单位1.Natl Deep Sea Ctr, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Peoples R China
5.Laoshan Lab, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
6.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Yan,Chen, Hao,Lian, Chao,et al. Insights into phage-bacteria interaction in cold seep Gigantidas platifrons through metagenomics and transcriptome analyses[J]. SCIENTIFIC REPORTS,2024,14(1):12.
APA Zhang, Yan.,Chen, Hao.,Lian, Chao.,Cao, Lei.,Guo, Yang.,...&Li, Chaolun.(2024).Insights into phage-bacteria interaction in cold seep Gigantidas platifrons through metagenomics and transcriptome analyses.SCIENTIFIC REPORTS,14(1),12.
MLA Zhang, Yan,et al."Insights into phage-bacteria interaction in cold seep Gigantidas platifrons through metagenomics and transcriptome analyses".SCIENTIFIC REPORTS 14.1(2024):12.

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来源：海洋研究所

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