Comparative assessment of the intragenomic variations of dinoflagellate Tripos species through single-cell sequencing
文献类型：期刊论文
| 作者 | Huang, Xianliang1,2,3,4; Li, Yingchao1,2,3,4; Du, Haina1,2,3,4; Chen, Nansheng2 |
| 刊名 | MARINE POLLUTION BULLETIN
 |
| 出版日期 | 2024-09-01 |
| 卷号 | 206页码:12 |
| 关键词 | Tripos Single cell analysis Intra-genomic variations (IGVs) Molecular diversity Metabarcoding |
| ISSN号 | 0025-326X |
| DOI | 10.1016/j.marpolbul.2024.116690 |
| 通讯作者 | Chen, Nansheng(chenn@qdio.ac.cn) |
| 英文摘要 | Tripos is a large dinoflagellate genus widely distributed in the world 's oceans. Morphology-based species identification is inconclusive due to high morphological intraspecific variability. Metabarcoding analysis has been demonstrated to be effective for species identification and tracking their spatiotemporal dynamics. However, accumulating evidence suggests high levels of intragenomic variations (IGVs) are common in many algae, leading to concerns about overinterpretation of molecular diversity in metabarcoding studies. In this project, we evaluated and compared IGVs in Tripos species by conducting the first high-throughput sequencing (HTS) of 18S rDNA V4 of Tripos single cells. High numbers of haplotypes (19 -172) were identified in each of the 30 Tripos cells. Each cell contained one dominant haplotype with high relative abundance and many haplotypes with lower abundances. Thus, the presence of multiple minor haplotypes substantially overestimate the molecular diversity identified in metabarcoding analysis, which encompass not only interspecific and intraspecific diversities, but high levels of IGVs. |
| WOS关键词 | INTERNAL TRANSCRIBED SPACER ; HARMFUL ALGAL BLOOMS ; RED-TIDE ; MOLECULAR DIVERSITY ; PROTIST DIVERSITY ; RARE BIOSPHERE ; CERATIUM FURCA ; RIBOSOMAL DNA ; GENUS TRIPOS ; RDNA OPERON |
| 资助项目 | Marine S&T Fund of Shandong Province for Pilot Qingdao National Laboratory for Marine Science and Technology[LSKJ202203700] ; National Key Research and Development Program of China[2022YFC3105200] ; Taishan Scholar Project Special Fund ; Natural Science Foundation of China[42176162] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000] ; Chinese Academy of Sciences Pioneer Hundred Talents Program |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001273705900001 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186275]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Chen, Nansheng |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100039, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Xianliang,Li, Yingchao,Du, Haina,et al. Comparative assessment of the intragenomic variations of dinoflagellate Tripos species through single-cell sequencing[J]. MARINE POLLUTION BULLETIN,2024,206:12. |
| APA | Huang, Xianliang,Li, Yingchao,Du, Haina,&Chen, Nansheng.(2024).Comparative assessment of the intragenomic variations of dinoflagellate Tripos species through single-cell sequencing.MARINE POLLUTION BULLETIN,206,12. |
| MLA | Huang, Xianliang,et al."Comparative assessment of the intragenomic variations of dinoflagellate Tripos species through single-cell sequencing".MARINE POLLUTION BULLETIN 206(2024):12. |
入库方式： OAI收割
来源：海洋研究所
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