Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus
文献类型：期刊论文
| 作者 | Xue, Dongxiu1,3 ; Xia, Sudong2; Xing, Tengfei1,3; Gao, Yuqian2; Liu, Jinxian1,3
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| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 出版日期 | 2024-07-06 |
| 页码 | 10 |
| 关键词 | Trachidermus fasciatus transcriptome ovary testis sex-related genes |
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-024-3285-8 |
| 通讯作者 | Xue, Dongxiu(xuedongxiu@qdio.ac.cn) |
| 英文摘要 | The mechanisms underlying sex determination and differentiation have long intrigued researchers in the fields of development and evolutionary biology. The roughskin sculpin (Trachidermus fasciatus Heckel), displaying sexual dimorphism, provides an ideal model for studying the mechanisms. However, both genetic and genomic information concerning sex determination and differentiation, such as gonadal transcriptome data in roughskin sculpin, are lacking. Here, we present the first gonadal transcriptomes of roughskin sculpin and identify sex-related genes. We identified 8 531 differentially expressed genes (DEGs), among them 4 065 were upregulated in the ovary and 4 466 upregulated in the testis. Several sex-related gene ontology (GO) terms were enriched in ovary-biased genes, including "binding of sperm to zona pellucida", "egg coat formation", "positive regulation of acrosome reaction", "cell division", and "cell cycle", while the GO terms such as "spermatogenesis", "sperm axoneme assembly", "cilium assembly", "cilium movement", and "cilium movement involved in cell motility" were enriched in testis-biased genes. Moreover, six KEGG pathways were significantly enriched in the ovary, whereas only one was enriched in the testis. Of these DEGs, 40 sex-related genes were identified, which including 26 testis-biased genes (such as Dmrtb1, Gsdf, Sox9b, Wnt4b, Tcp11l2, and Efhb), and 14 ovary-biased genes (such as Cyp19a1a, Foxh1, Foxr1, Gdf3, Hsd17b12, and Igf2bp3). This gonadal transcript dataset would broaden our understanding of sex determination and differentiation mechanisms in roughskin sculpin, expand the genomic database, support future studies on sex-related gene functions, and facilitate molecular biology research into roughskin sculpin. |
| WOS关键词 | WNT4 EXPRESSION ; DIFFERENTIATION ; HISAT ; SOX9 |
| 资助项目 | National Natural Science Foundation of China[31972793] ; National Natural Science Foundation of China[31502169] ; Key Scientific Research Project in Universities and Colleges in Tianjin[2022ZD004] |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:001263497800001 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186290]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Xue, Dongxiu |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 2.Dept Fishery Sci, Tianjin Key Lab Aqua Ecol & Aquaculture, Tianjin 300380, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Dongxiu,Xia, Sudong,Xing, Tengfei,et al. Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2024:10. |
| APA | Xue, Dongxiu,Xia, Sudong,Xing, Tengfei,Gao, Yuqian,&Liu, Jinxian.(2024).Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,10. |
| MLA | Xue, Dongxiu,et al."Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2024):10. |
入库方式： OAI收割
来源：海洋研究所
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