Study on the Characteristics and Mechanism of Inorganic Nitrogen Nutrients Preferential Assimilation by Phaeocystis globosa
文献类型：期刊论文
| 作者 | Guo, Honghong1,2,3; Wang, Wentao1,2,4 ; Han, Jishu3; Zhu, Jianan1,2; Wang, Zhongshi1,2,4; He, Liyan1,2; Song, Xiuxian1,2,4; Yu, Zhiming1,2,4
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| 刊名 | JOURNAL OF MARINE SCIENCE AND ENGINEERING
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| 出版日期 | 2024-06-01 |
| 卷号 | 12期号:6页码:15 |
| 关键词 | Phaeocystis globosa nitrate ammonium isotope tracer assimilation |
| DOI | 10.3390/jmse12060989 |
| 通讯作者 | Wang, Wentao(wtwang@qdio.ac.cn) |
| 英文摘要 | Phaeocystis globosa is an important harmful algal species that is globally distributed. Previous studies have indicated that P. globosa preferentially uptake nitrate, but the underlying mechanism is still unclear. To further verify this preference and reveal the underlying mechanism, the assimilation rates of nitrate and ammonium by P. globosa at different concentrations was quantitatively studied by using a nitrogen stable isotope (N-15) tracer technique, and the regulatory mechanism was determined from the physiological and biochemical responses. The findings revealed that the preferential assimilation of nitrate by P. globosa was influenced by the ambient ammonium concentration. When the ambient concentration of ammonium was less than approximately 3.5 mu mol center dot L-1, the assimilation rates of nitrate form P. globosa were as high as 1.05 x 10(-5) mu mol center dot L-1 center dot h(-1)center dot cell(-1). Even though the nitrate assimilation in P. globosa was inhibited at ammonium concentrations greater than this threshold, nitrate assimilation could not be completely suppressed. The activity of NR and the expression of related genes in P. globosa were also affected by ammonium. In addition, N-15 signals originally labeled nitrate accumulated in ammonium. This indicated that P. globosa was likely to reduce excess nitrate to ammonium and subsequently release it into the substrate, which might be an important energy dissipation mechanism for P. globosa. These results expand the classical understanding of the utilization of nitrogen nutrients by marine phytoplankton, and offer valuable resources for comprehending the mechanism of harmful algal blooms. |
| WOS关键词 | NITRATE UPTAKE ; SOUTHERN BIGHT ; NORTH-SEA ; PHYTOPLANKTON ; AMMONIUM ; GROWTH ; INHIBITION ; DYNAMICS ; KINETICS ; RELEASE |
| 资助项目 | Shandong Provincial Natural Science Foundation of China[ZR2019QD014] ; Intergovernmental Innovation Cooperation Project of the Ministry of Science and Technology[2022YFE0136400] ; Laoshan Laboratory[LSKJ202203700] ; National Natural Science Foundation of China[42306150] |
| WOS研究方向 | Engineering ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:001257064600001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186443]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Wang, Wentao |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Honghong,Wang, Wentao,Han, Jishu,et al. Study on the Characteristics and Mechanism of Inorganic Nitrogen Nutrients Preferential Assimilation by Phaeocystis globosa[J]. JOURNAL OF MARINE SCIENCE AND ENGINEERING,2024,12(6):15. |
| APA | Guo, Honghong.,Wang, Wentao.,Han, Jishu.,Zhu, Jianan.,Wang, Zhongshi.,...&Yu, Zhiming.(2024).Study on the Characteristics and Mechanism of Inorganic Nitrogen Nutrients Preferential Assimilation by Phaeocystis globosa.JOURNAL OF MARINE SCIENCE AND ENGINEERING,12(6),15. |
| MLA | Guo, Honghong,et al."Study on the Characteristics and Mechanism of Inorganic Nitrogen Nutrients Preferential Assimilation by Phaeocystis globosa".JOURNAL OF MARINE SCIENCE AND ENGINEERING 12.6(2024):15. |
入库方式： OAI收割
来源：海洋研究所
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