Evaluating the effectiveness of the eRNA technique in monitoring fish biodiversity - A case study in the Qingdao offshore, China
文献类型：期刊论文
| 作者 | Li, Wenqiong1,2 ; Jia, Hui1,3; Zhang, Hui1,2
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| 刊名 | GLOBAL ECOLOGY AND CONSERVATION
 |
| 出版日期 | 2024-06-01 |
| 卷号 | 51页码:17 |
| 关键词 | eRNA technique eDNA technique Fish biodiversity Qingdao offshore |
| DOI | 10.1016/j.gecco.2024.e02888 |
| 通讯作者 | Zhang, Hui(zhanghui@qdio.ac.cn) |
| 英文摘要 | With the continuous intensification of human activities, fish biodiversity has been reduced in the Yellow Sea. Therefore, accurate monitoring methods are crucial for determining fish biodiversity in the target area. The environmental DNA (eDNA) technique has emerged as a promising noninvasive method, but it is prone to false positives. The rapid degradation of environmental RNA (eRNA) has the potential to reduce false positives in the eDNA technique; however, there is limited research on using the eRNA technique to study fish biodiversity. This study aimed to explore a suitable sampling method for extracting sufficient eRNA from seawater samples. Furthermore, we performed a case study in Qingdao offshore, China, to survey fish species and community composition based on the eRNA and eDNA techniques in November 2022 using seawater samples collected from 19 sampling sites. We compared the results with data obtained through traditional field survey (TFS) methods and examined the relationship between fish species and environmental factors. The results revealed a significant overlap between fish species detected by the eRNA and eDNA techniques, with the eRNA technique demonstrating higher sensitivity and positive predictivity, and fewer false positives compared to those of eDNA. Our study highlighted the potential of the eRNA technique as a powerful tool for improving the detection rate of fish species in marine ecosystems. |
| WOS关键词 | ENVIRONMENTAL DNA ; DEGRADATION ; DIVERSITY |
| 资助项目 | National Key Research and Development Project of China[2022YFE0112800] ; Taishan Scholars Program ; Youth Innovation Promotion Association CAS[2020211] ; Oceano-graphic Data Center, IOCAS |
| WOS研究方向 | Biodiversity & Conservation ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:001258492700001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186467]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Zhang, Hui |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ningbo Univ, Sch Marine Sci, Ningbo 315823, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wenqiong,Jia, Hui,Zhang, Hui. Evaluating the effectiveness of the eRNA technique in monitoring fish biodiversity - A case study in the Qingdao offshore, China[J]. GLOBAL ECOLOGY AND CONSERVATION,2024,51:17. |
| APA | Li, Wenqiong,Jia, Hui,&Zhang, Hui.(2024).Evaluating the effectiveness of the eRNA technique in monitoring fish biodiversity - A case study in the Qingdao offshore, China.GLOBAL ECOLOGY AND CONSERVATION,51,17. |
| MLA | Li, Wenqiong,et al."Evaluating the effectiveness of the eRNA technique in monitoring fish biodiversity - A case study in the Qingdao offshore, China".GLOBAL ECOLOGY AND CONSERVATION 51(2024):17. |
入库方式： OAI收割
来源：海洋研究所
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