Complementary environmental analysis and functional characterization of lower glycolysis-gluconeogenesis in the diatom plastid
文献类型：期刊论文
| 作者 | Dorrell, Richard G.1,10,11; Zhang, Youjun7,8,9; Liang, Yue5,6; Gueguen, Nolwenn4; Nonoyama, Tomomi3,11; Croteau, Dany2; Penot-Raquin, Mathias1,10,11; Adiba, Sandrine11; Bailleul, Benjamin2; Gros, Valerie4 |
| 刊名 | PLANT CELL
 |
| 出版日期 | 2024-06-06 |
| 页码 | 27 |
| ISSN号 | 1040-4651 |
| DOI | 10.1093/plcell/koae168 |
| 通讯作者 | Dorrell, Richard G.(richard.dorrell@sorbonne-universite.fr) |
| 英文摘要 | Organic carbon fixed in chloroplasts through the Calvin-Benson-Bassham Cycle can be diverted toward different metabolic fates, including cytoplasmic and mitochondrial respiration, gluconeogenesis, and synthesis of diverse plastid metabolites via the pyruvate hub. In plants, pyruvate is principally produced via cytoplasmic glycolysis, although a plastid-targeted lower glycolytic pathway is known to exist in non-photosynthetic tissue. Here, we characterized a lower plastid glycolysis-gluconeogenesis pathway enabling the direct interconversion of glyceraldehyde-3-phosphate and phospho-enol-pyruvate in diatoms, ecologically important marine algae distantly related to plants. We show that two reversible enzymes required to complete diatom plastid glycolysis-gluconeogenesis, Enolase and bis-phosphoglycerate mutase (PGAM), originated through duplications of mitochondria-targeted respiratory isoforms. Through CRISPR-Cas9 mutagenesis, integrative 'omic analyses, and measured kinetics of expressed enzymes in the diatom Phaeodactylum tricornutum, we present evidence that this pathway diverts plastid glyceraldehyde-3-phosphate into the pyruvate hub, and may also function in the gluconeogenic direction. Considering experimental data, we show that this pathway has different roles dependent in particular on day length and environmental temperature, and show that the cpEnolase and cpPGAM genes are expressed at elevated levels in high-latitude oceans where diatoms are abundant. Our data provide evolutionary, meta-genomic, and functional insights into a poorly understood yet evolutionarily recurrent plastid metabolic pathway. Environmental and experimental methods reveal the importance of plastid-targeted Enolase and bis-phosphoglycerate mutase enzymes to diatoms, marine algae that contribute to global primary production. |
| WOS关键词 | MARINE DIATOM ; PREDICTION ; ACID ; TOOL ; LOCALIZATION ; ORGANIZATION ; EVOLUTION ; BIOSYNTHESIS ; ASSIMILATION ; EXPRESSION |
| 资助项目 | Agence Nationale de la Recherche (ANR) Jeune Chercheur/Jeune Chercheuse (JCJC) from 2021 to 2022[ANR-21-CE02-0014-01] ; European Research Council (ERC) Starting Grant (ChloroMosaic) from 2023 to 2027[101039760] ; Fonds Francais de l'Environnement Mondial (FFEM)/French Facility for Global Environment ; French Government Investissements d'Avenir programs OCEANOMICS[ANR-11-BTBR-0008] ; Organisation et Montee en Puissance d'une Infrastructure Nationale de Genomique (FRANCE GENOMIQUE)[ANR-10-INBS-0009] ; Memory in Living Systems: An Integrative Approach (MEMOLIFE)[ANR-10-LABX-0054] ; Paris Sciences et Lettres (PSL) Research University[ANR-11-IDEX-0001-02] ; European Research Council (ERC) research and innovation program (Diatomic)[835067] ; ANR BrownCut project[ANR-19-CE20-0020] ; ANR DIM[ANR-21-CE02-0021] ; Programmes et Equipements Prioritaires de la Recherche (PEPR) Novel Strategies for the Development of Microalgae as Renewable Biofuel Resources (AlgAdvance)[A-22-PEBB-0002] ; European Regional Development Fund (ERDF) ; Labex GRAL[10-LABX-0049] ; University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) Chimie-Biologie-Sante CBH-EUR-GS[A-17-EURE-0003] ; European Research Council (ERC) research and innovation program[739582] ; European Research Council (ERC) research and innovation program[66462] ; European Regional Development Fund through the Bulgarian Science and Education for Smart Growth Operational Programme[BG05M2OP001-1.003-001-C01] ; European Research Council (ERC) under the research and innovation program (PhotoPHYTOMIX project)[715579] ; Centre National de la Recherche Scientifique (CNRS) Momentum Fellowship (The Carbon Cycle Revisited) 2019 to 2021 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001255673200001 |
| 出版者 | OXFORD UNIV PRESS INC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/186404]  |
| 专题 | 海洋研究所_深海极端环境与生命过程研究中心 |
| 通讯作者 | Dorrell, Richard G. |
| 作者单位 | 1.CNRS, Res Federat Study Global Ocean Syst Ecol & Evolut, Tara Oceans GOSEE FR2022, F-75016 Paris, France 2.Univ PSL, Inst Biol Physico Chim IBPC, F-75005 Paris, France 3.Tokyo Univ Agr & Technol, Inst Engn, Div Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan 4.Univ Grenoble Alpes, CNRS, CEA, INRAE,IRIG,Lab Physiol Cellulaire & Vegetale, F-38000 Grenoble, France 5.Pilot Natl Lab Marine Sci & Technol, Lab Marine Mineral Resources, Qingdao 266237, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 7.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Seed Innovat, Beijing 100101, Peoples R China 8.Max Planck Inst Mol Plant Physiol, Cent Plant Metab Grp, D-14476 Potsdam, Germany 9.Ctr Plant Syst Biol & Biotechnol, Dept Plant Metabol, Plovdiv 4000, Bulgaria 10.Sorbonne Univ, INSERM, CNRS, Inst Biol Paris Seine IBPS,Lab Computat & Quantita, F-75005 Paris, France |
| 推荐引用方式 GB/T 7714 | Dorrell, Richard G.,Zhang, Youjun,Liang, Yue,et al. Complementary environmental analysis and functional characterization of lower glycolysis-gluconeogenesis in the diatom plastid[J]. PLANT CELL,2024:27. |
| APA | Dorrell, Richard G..,Zhang, Youjun.,Liang, Yue.,Gueguen, Nolwenn.,Nonoyama, Tomomi.,...&Bowler, Chris.(2024).Complementary environmental analysis and functional characterization of lower glycolysis-gluconeogenesis in the diatom plastid.PLANT CELL,27. |
| MLA | Dorrell, Richard G.,et al."Complementary environmental analysis and functional characterization of lower glycolysis-gluconeogenesis in the diatom plastid".PLANT CELL (2024):27. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。