中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 自动化研究所
  3. 中国科学院自动化研究所
  4. 中国科学院分子影像重点实验室
Adhesion molecule-targeted magnetic particle imaging nanoprobe for visualization of inflammation in acute lung injury

文献类型：期刊论文

作者Gao, Pengli1,2,3,4; Liu, Yu1,2,3,4; Wang, Xiaoli5; Feng, Xin4; Liu, Heng6; Liu, Songlu4; Huang, Xiazi4; Wu, Xiangjun1,2,3,4; Xiong, Fei1,2,3,4; Jia, Xiaohua4
刊名EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
出版日期2023-12-14
页码13
关键词Acute lung injury Inflammation Vascular cell adhesion molecule-1 Magnetic particle imaging
ISSN号1619-7070
DOI10.1007/s00259-023-06550-4
通讯作者Hui, Hui(hui.hui@ia.ac.cn) ; Jiang, Jingying(jingyingjiang@buaa.edu.cn) ; Tian, Jie(jie.tian@ia.ac.cn)
英文摘要Purpose Uncontrolled intra-alveolar inflammation is a central pathogenic feature, and its severity translates into a valid prognostic indicator of acute lung injury (ALI). Unfortunately, current clinical imaging approaches are unsuitable for visualizing and quantifying intra-alveolar inflammation. This study aimed to construct a small-sized vascular cell adhesion molecule-1 (VCAM-1)-targeted magnetic particle imaging (MPI) nanoprobe (ESPVPN) to visualize and accurately quantify intra-alveolar inflammation at the molecular level. Methods ESPVPN was engineered by conjugating a peptide (VHPKQHRGGSK(Cy7)GC) onto a polydopamine-functionalized superparamagnetic iron oxide core. The MPI performance, targeting, and biosafety of the ESPVPN were characterized. VCAM-1 expression in HUVECs and mouse models was evaluated by western blot. The degree of inflammation and distribution of VCAM-1 in the lungs were assessed using histopathology. The expression of pro-inflammatory markers and VCAM-1 in lung tissue lysates was measured using ELISA. After intravenous administration of ESPVPN, MPI and CT imaging were used to analyze the distribution of ESPVPN in the lungs of the LPS-induced ALI models. Results The small-sized (similar to 10 nm) ESPVPN exhibited superior MPI performance compared to commercial MagImaging((R)) and Vivotrax, and ESPVPN had effective targeting and biosafety. VCAM-1 was highly expressed in LPS-induced ALI mice. VCAM-1 expression was positively correlated with the LPS-induced dose (R = 0.9381). The in vivo MPI signal showed positive correlations with both VCAM-1 expression (R = 0.9186) and representative pro-inflammatory markers (MPO, TNF-alpha, IL-6, IL-8, and IL-1 beta, R > 0.7). Conclusion ESPVPN effectively targeted inflammatory lungs and combined the advantages of MPI quantitative imaging to visualize and evaluate the degree of ALI inflammation.
WOS关键词RESPIRATORY-DISTRESS-SYNDROME ; QUANTIFICATION ; MORTALITY ; TRACKING
资助项目National Natural Science Foundation of China ; National Research Facility for Phenotypic and Genetic Analysis of Model Animals
WOS研究方向Radiology, Nuclear Medicine & Medical Imaging
语种英语
WOS记录号WOS:001125141400002
出版者SPRINGER
资助机构National Natural Science Foundation of China ; National Research Facility for Phenotypic and Genetic Analysis of Model Animals
源URL[http://ir.ia.ac.cn/handle/173211/58638]  
专题自动化研究所_中国科学院分子影像重点实验室
通讯作者Hui, Hui; Jiang, Jingying; Tian, Jie
作者单位1.Beihang Univ, Sch Biol Sci & Med Engn, 37 Xueyuan Rd, Beijing 100191, Peoples R China
2.Beihang Univ, Sch Engn Med, 37 Xueyuan Rd, Beijing 100191, Peoples R China
3.Beihang Univ, Key Lab Big Data Based Precis Med, Minist Ind & Informat Technol Peoples Republ China, 37 Xueyuan Rd, Beijing 100191, Peoples R China
4.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Key Lab Mol Imaging, Beijing 100190, Peoples R China
5.Weifang Med Univ, Sch Med Imaging, Weifang 261053, Peoples R China
6.PLA Rocket Force Characterist Med Ctr, Dept Radiol, 16 Xinjiekou Outer St, Beijing 100088, Peoples R China
推荐引用方式
GB/T 7714		 Gao, Pengli,Liu, Yu,Wang, Xiaoli,et al. Adhesion molecule-targeted magnetic particle imaging nanoprobe for visualization of inflammation in acute lung injury[J]. EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,2023:13.
APA Gao, Pengli.,Liu, Yu.,Wang, Xiaoli.,Feng, Xin.,Liu, Heng.,...&Tian, Jie.(2023).Adhesion molecule-targeted magnetic particle imaging nanoprobe for visualization of inflammation in acute lung injury.EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,13.
MLA Gao, Pengli,et al."Adhesion molecule-targeted magnetic particle imaging nanoprobe for visualization of inflammation in acute lung injury".EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING (2023):13.

入库方式： OAI收割

来源：自动化研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。