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Peak Shift in Field-Swept Magnetic Particle Spectroscopy: A Novel Sensing Metric for Magnetic Nanoparticle Bioassays

文献类型：期刊论文

作者Liu, Yanjun1,2,3; Feng, Xin4,5,6; Liu, Sijia7; Li, Lei8; Huang, Xing-Yao9; He, Mengjiao9; Qin, Cheng-Feng9; Hui, Hui4,5,6,10; Tian, Jie1,2,3,10
刊名IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
出版日期2024
卷号73页码:10
关键词Harmonic analysis Coronaviruses Magnetic fields Magnetization Hydrodynamics Power harmonic filters Coils Magnetic nanoparticles (MNPs) magnetic particle spectroscopy (MPS) point-of-care (PoC) diagnostics second harmonic
ISSN号0018-9456
DOI10.1109/TIM.2024.3436100
通讯作者Feng, Xin(xin.feng@ia.ac.cn) ; Tian, Jie(tian@ieee.org)
英文摘要Combining magnetic particle spectroscopy (MPS) with functionalized magnetic nanoparticles (MNPs) has the potential to provide a rapid, sensitive, and user-friendly point-of-care (PoC) testing platform. Conventional MPS techniques typically apply harmonic ratios (such as, the third to fifth harmonic ratio) as the sensing metric to eliminate interference caused by MNP concentration. However, the quantification accuracy of this approach largely depends on the consistency of the signal-to-noise ratio (SNR) between the selected harmonics, imposing stringent requirements on hardware design and the nonlinear magnetic behavior of the MNPs. In this study, we introduce "peak shift" to field-swept MPS (FS-MPS) as a novel metric for MNP characterization and biosensing. An integrated FS-MPS device is used to capture the second harmonic spectrum, which records the second harmonic amplitudes under different perpendicular static magnetic fields. Importantly, the peak position of the spectrum remains constant under different MNP concentrations and shifts upon changes in MNP hydrodynamic size. In this way, the peak shift sensitively detects MNP binding to the targeted analytes, enabling robust biosensing based solely on the second harmonics. The feasibility of the proposed method was demonstrated using streptavidin-grafted MNPs and biotinylated polystyrene beads. Diagnostic performance was assessed using ACE2 functionalized MNPs and SARS-CoV-2 S1 protein and SARS-CoV-2 pseudovirus assays. The proposed strategy can detect virus concentrations as low as 350 TCID50/mL. Additionally, a good linear correlation exists between the peak shift and virus titer in the 350-7000 TCID50/mL range, demonstrating the potential of our method to quantify virus content.
WOS关键词SARS-COV-2
资助项目National Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[81930053] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[82302407] ; National Natural Science Foundation of China[32350010] ; National Natural Science Foundation of China[92359304] ; National Natural Science Foundation of China[U23A6011] ; Beijing Natural Science Foundation[L234056] ; Beijing Natural Science Foundation[7232346] ; Beijing Natural Science Foundation[JQ22023] ; Chinese Academy of Sciences (CAS) Youth Innovation Promotion Association[Y2022055]
WOS研究方向Engineering ; Instruments & Instrumentation
语种英语
WOS记录号WOS:001288455200013
出版者IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Chinese Academy of Sciences (CAS) Youth Innovation Promotion Association
源URL[http://ir.ia.ac.cn/handle/173211/59303]  
专题自动化研究所_中国科学院分子影像重点实验室
通讯作者Feng, Xin; Tian, Jie
作者单位1.Beihang Univ, Sch Engn Med, Beijing 100191, Peoples R China
2.Beihang Univ, Sch Biol Sci & Med Engn, Beijing 100191, Peoples R China
3.Minist Ind & Informat Technol China, Key Lab Big Data Based Precis Med, Beijing 100191, Peoples R China
4.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
5.Beijing Key Lab Mol Imaging, Beijing 100190, Peoples R China
6.Univ Chinese Acad Sci, Sch Artificial Intelligence, Beijing 100080, Peoples R China
7.Southeast Univ, Sch Comp Sci & Engn, Nanjing 210096, Peoples R China
8.Xidian Univ, Sch Life Sci & Technol, Xian 710126, Peoples R China
9.Acad Mil Med Sci, Dept Virol, State Key Lab Pathogen & Biosecur, Beijing Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China
10.Natl Key Lab Kidney Dis, Beijing 100853, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Yanjun,Feng, Xin,Liu, Sijia,et al. Peak Shift in Field-Swept Magnetic Particle Spectroscopy: A Novel Sensing Metric for Magnetic Nanoparticle Bioassays[J]. IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT,2024,73:10.
APA Liu, Yanjun.,Feng, Xin.,Liu, Sijia.,Li, Lei.,Huang, Xing-Yao.,...&Tian, Jie.(2024).Peak Shift in Field-Swept Magnetic Particle Spectroscopy: A Novel Sensing Metric for Magnetic Nanoparticle Bioassays.IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT,73,10.
MLA Liu, Yanjun,et al."Peak Shift in Field-Swept Magnetic Particle Spectroscopy: A Novel Sensing Metric for Magnetic Nanoparticle Bioassays".IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT 73(2024):10.

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来源：自动化研究所

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