Lasiokaurin Regulates PLK1 to Induce Breast Cancer Cell G2/M Phase Block and Apoptosis
文献类型：期刊论文
| 作者 | Liu, Zhengrui3,4; Wang, Jia3,4; Xie, Siman3,4; Zhang, Benteng3,4; Yuan, Yan3,4; Fu, Huaizi3,4; Hao, Hongyun3,4; Sun, Li3,4; Yuan, Shengtao3,4; Ding, Jian2
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| 刊名 | JOURNAL OF CANCER
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| 出版日期 | 2024 |
| 卷号 | 15期号:8页码:2318-2328 |
| 关键词 | Lasiokaurin breast cancer G2/M phase block apoptosis PLK1 |
| ISSN号 | 1837-9664 |
| DOI | 10.7150/jca.93621 |
| 通讯作者 | Ding, Jian(jding@simm.ac.cn) ; Yu, Hong(yuhong@njmu.edu.cn) ; Yang, Mei(yangmei@cpu.edu.cn) |
| 英文摘要 | Aim of the study: To investigate the anti -tumor effects of Lasiokaurin on breast cancer and explore its underlying molecular mechanism. Materials and methods: In this study, MTT assay, plate colony formation assays, soft agar assay, and EdU assay were employed to evaluate the anti -proliferation effects of LAS. Apoptosis and cell cycle distribution were detected by flow cytometry. The molecular mechanism was predicted by performing RNA sequencing and verified by using immunoblotting assays. Breast cancer organiods derived from patient -derived xenografts model and MDA-MB-231 xenograft mouse model were established to assess the effect of LAS. Results: Our study showed that LAS treatment significantly suppressed cell viability of 5 breast cancer cell lines, with the IC 50 value of approximately 1-5 mu M. LAS also inhibitied the clonogenic ability and DNA synthesis of breast cancer cells, Moreover, LAS induced apoptosis and G2/M cell cycle arrest in SK -BR -3 and MDA-MB-231 cells. Notably, transcriptomic analysis predicted the mechanistic involvement of PLK1 in LAS -suppressed breast cancer progression. Our experiment data further verified that LAS reduced PLK1 mRNA and protein expression in breast cancer, accompanied by downregulating CDC25C and AKT phosphorylation. Ultimately, we confirmed that LAS inhibit breast cancer growth via inhibiting PLK1 pathway in vivo. Conclusions: Collectively, our findings revealed that LAS inhibits breast cancer progression via regulating PLK1 pathway, which provids scientific evidence for the use of traditional Chinese medicine in cancer therapy. |
| WOS关键词 | KINASE 1 ; DITERPENOIDS ; CYCLE ; PHOSPHORYLATION ; INHIBITION ; STATISTICS ; RUBESCENS ; PROTEIN |
| 资助项目 | Jiangsu Funding Program for Excellent Postdoctoral Talent[1412200065] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:001255874700017 |
| 出版者 | IVYSPRING INT PUBL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/312044]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ding, Jian; Yu, Hong; Yang, Mei |
| 作者单位 | 1.Nanjing Med Univ, Affiliated Taizhou Peoples Hosp, Dept Pathol, Taizhou, Jiangsu, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 3.China Pharmaceut Univ, Natl Key Lab Multitarget Nat Drugs, Nanjing, Peoples R China 4.China Pharmaceut Univ, Jiangsu Key Lab Drug Screening, Nanjing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhengrui,Wang, Jia,Xie, Siman,et al. Lasiokaurin Regulates PLK1 to Induce Breast Cancer Cell G2/M Phase Block and Apoptosis[J]. JOURNAL OF CANCER,2024,15(8):2318-2328. |
| APA | Liu, Zhengrui.,Wang, Jia.,Xie, Siman.,Zhang, Benteng.,Yuan, Yan.,...&Yang, Mei.(2024).Lasiokaurin Regulates PLK1 to Induce Breast Cancer Cell G2/M Phase Block and Apoptosis.JOURNAL OF CANCER,15(8),2318-2328. |
| MLA | Liu, Zhengrui,et al."Lasiokaurin Regulates PLK1 to Induce Breast Cancer Cell G2/M Phase Block and Apoptosis".JOURNAL OF CANCER 15.8(2024):2318-2328. |
入库方式： OAI收割
来源：上海药物研究所
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