LPS exacerbates TRPV4-mediated itch through the intracellular TLR4-PI3K signalling
文献类型：期刊论文
| 作者 | Hao, Yanping4,5,6; Wu, Liyan5,6; Wang, Yuhui3; Shan, Dongmei5,6; Liu, Yifei6; Feng, Jing5,6; Chang, Yi4; Wang, Ting1,2,5,6 |
| 刊名 | JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
 |
| 出版日期 | 2024-07-01 |
| 卷号 | 28期号:13页码:9 |
| 关键词 | itch sensitization LPS PI3K TLR4 TRPV4 |
| ISSN号 | 1582-1838 |
| DOI | 10.1111/jcmm.18509 |
| 通讯作者 | Feng, Jing(fengjing@simm.ac.cn) ; Chang, Yi(chani_1213@163.com) ; Wang, Ting(wangting@simm.ac.cn) |
| 英文摘要 | Pruritus is often accompanied with bacterial infections, but the underlying mechanism is not fully understood. Although previous studies revealed that lipopolysaccharides (LPS) could directly activate TRPV4 channel and TRPV4 is involved in the generation of both acute itch and chronic itch, whether and how LPS affects TRPV4-mediated itch sensation remains unclear. Here, we showed that LPS-mediated TRPV4 sensitization exacerbated GSK101-induced scratching behaviour in mice. Moreover, this effect was compromised in TLR4-knockout mice, suggesting LPS acted through a TLR4-dependent mechanism. Mechanistically, LPS enhanced GSK101-evoked calcium influx in mouse ear skin cells and HEK293T cells transfected with TRPV4. Further, LPS sensitized TRPV4 channel through the intracellular TLR4-PI3K-AKT signalling. In summary, our study found a modulatory role of LPS in TRPV4 function and highlighted the TLR4-TRPV4 interaction in itch signal amplification. |
| WOS关键词 | LIPOPOLYSACCHARIDE ; ACTIVATION ; PROTECTS ; CALCIUM |
| 资助项目 | Research Project of Yunnan Key Laboratory of Gastrodia and Fungi Symbiotic Biology |
| WOS研究方向 | Cell Biology ; Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:001261132400001 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/312104]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Feng, Jing; Chang, Yi; Wang, Ting |
| 作者单位 | 1.Zhaotong Univ, Yunnan Engn Res Ctr Green Planting & Proc Gastrodi, Zhaotong, Yunnan, Peoples R China 2.Zhaotong Univ, Yunnan Key Lab Gastrodia & Fungi Symbiot Biol, Zhaotong, Yunnan, Peoples R China 3.Chinese Acad Med Sci & Peking Union Med Coll, Plast Surg Hosp, Dept Anesthesiol, Beijing, Peoples R China 4.Tongji Univ, Yangpu Hosp, Sch Med, Shanghai 200090, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Yanping,Wu, Liyan,Wang, Yuhui,et al. LPS exacerbates TRPV4-mediated itch through the intracellular TLR4-PI3K signalling[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2024,28(13):9. |
| APA | Hao, Yanping.,Wu, Liyan.,Wang, Yuhui.,Shan, Dongmei.,Liu, Yifei.,...&Wang, Ting.(2024).LPS exacerbates TRPV4-mediated itch through the intracellular TLR4-PI3K signalling.JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,28(13),9. |
| MLA | Hao, Yanping,et al."LPS exacerbates TRPV4-mediated itch through the intracellular TLR4-PI3K signalling".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 28.13(2024):9. |
入库方式： OAI收割
来源：上海药物研究所
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