A New Electron Shuttling Pathway Mediated by Lipophilic Phenoxazine via the Interaction with Periplasmic and Inner Membrane Proteins of Shewanella oneidensis MR-1
文献类型：期刊论文
| 作者 | Wu, Yundang5; Zhu, Xiao1,4,5; Wang, Xinxin3,5; Lin, Zhixin5; Reinfelder, John R.2; Li, Fangbai5; Liu, Tongxu5 |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2023 |
| 卷号 | 57期号:6页码:2636-2646 |
| 关键词 | C-TYPE CYTOCHROMES ANAEROBIC RESPIRATION REDUCTION FERMENTATION INVOLVEMENT MECHANISMS DEPENDENCE GEOBACTER ACCEPTORS QUINONES |
| ISSN号 | 0013-936X |
| DOI | 10.1021/acs.est.2c07862 |
| 英文摘要 | Although it has been established that electron mediators substantially promote extracellular electron transfer (EET), electron shuttling pathways are not fully understood. Here, a new electron shuttling pathway was found in the EET process by Shewanella oneidensis MR-1 with resazurin, a lipophilic electron mediator. With resazurin, the genes encoding outer-membrane cytochromes (mtrCBA and omcA) were downregulated. Although cytochrome deletion substantially reduced biocurrent generation to 1-12% of that of wild-type (WT) cells, the presence of resazurin restored biocurrent generation to 168 μA·cm-2(ΔmtrA/omcA/mtrC), nearly equivalent to that of WT cells (194 μA·cm-2), indicating that resazurin-mediated electron transfer was not dependent on the Mtr pathway. Biocurrent generation by resazurin was much lower in ΔcymA and ΔmtrA/omcA/mtrC/fccA/cctA mutants (4 and 6 μA·cm-2) than in WT cells, indicating a key role of FccA, CctA, and CymA in this process. The effectiveness of resazurin in EET of Mtr cytochrome mutants is also supported by cyclic voltammetry, resazurin reduction kinetics, and in situ c-type cytochrome spectroscopy results. The findings demonstrated that low molecular weight, lipophilic electron acceptors, such as phenoxazine and phenazine, may facilitate electron transfer directly from periplasmic and inner membrane proteins, thus providing new insight into the roles of exogenous electron mediators in electron shuttling in natural and engineered biogeochemical systems. ? 2023 American Chemical Society. All rights reserved. |
| WOS研究方向 | Engineering, Environmental ; Environmental Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000920252300001 |
| 源URL | [http://ir.gig.ac.cn/handle/344008/80251]  |
| 专题 | 中国科学院广州地球化学研究所 |
| 作者单位 | 1.University of Chinese Academy of Sciences, Beijing; 100049, China 2.Department of Environmental Sciences, Rutgers University, New Brunswick; NJ; 08901, United States 3.College of Materials and Energy, South China Agricultural University, Guangzhou; 510642, China 4.Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou; 510640, China 5.Natl.-Reg. Jt. Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou; 510650, China |
| 推荐引用方式 GB/T 7714 | Wu, Yundang,Zhu, Xiao,Wang, Xinxin,et al. A New Electron Shuttling Pathway Mediated by Lipophilic Phenoxazine via the Interaction with Periplasmic and Inner Membrane Proteins of Shewanella oneidensis MR-1[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2023,57(6):2636-2646. |
| APA | Wu, Yundang.,Zhu, Xiao.,Wang, Xinxin.,Lin, Zhixin.,Reinfelder, John R..,...&Liu, Tongxu.(2023).A New Electron Shuttling Pathway Mediated by Lipophilic Phenoxazine via the Interaction with Periplasmic and Inner Membrane Proteins of Shewanella oneidensis MR-1.ENVIRONMENTAL SCIENCE & TECHNOLOGY,57(6),2636-2646. |
| MLA | Wu, Yundang,et al."A New Electron Shuttling Pathway Mediated by Lipophilic Phenoxazine via the Interaction with Periplasmic and Inner Membrane Proteins of Shewanella oneidensis MR-1".ENVIRONMENTAL SCIENCE & TECHNOLOGY 57.6(2023):2636-2646. |
入库方式： OAI收割
来源：广州地球化学研究所
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