Meta-omics elucidates key degraders in a bacterial tris(2-butoxyethyl) phosphate (TBOEP)-degrading enrichment culture
文献类型：期刊论文
| 作者 | Liang, Yi2,3; Zhou, Xiangyu1,2,3; Wu, Yiding1,2,3; Wu, Yang2,3; Zeng, Xiangying2,3; Yu, Zhiqiang2,3; Peng, Ping'an2,3 |
| 刊名 | WATER RESEARCH
 |
| 出版日期 | 2023 |
| 卷号 | 233页码:119774 |
| 关键词 | ORGANOPHOSPHORUS FLAME RETARDANTS IN-VITRO METABOLISM HUMAN LIVER EXPOSURE ETHER PLASTICIZERS ESTERS GENES BIOTRANSFORMATION BIOACCUMULATION |
| ISSN号 | 0043-1354 |
| DOI | 10.1016/j.watres.2023.119774 |
| 英文摘要 | Organophosphate esters (OPEs) are emerging contaminants of growing concern, and there is limited information about the bacterial transformation of OPEs. In this study, we investigated the biotransformation of tris(2-butoxyethyl) phosphate (TBOEP), a frequently detected alkyl-OPE by a bacterial enrichment culture under aerobic conditions. The enrichment culture degraded 5 mg/L TBOEP following the first-order kinetics with a reaction rate constant of 0.314 h?1. TBOEP was mainly degraded via ether bond cleavage, evidenced by the production of bis(2-butoxyethyl) hydroxyethyl phosphate, 2-butoxyethyl bis(2-hydroxyethyl) phosphate, and 2-butoxyethyl (2-hydroxyethyl) hydrogen phosphate. Other transformation pathways include terminal oxidation of the butoxyethyl group and phosphoester bond hydrolysis. Metagenomic sequencing generated 14 metagenome-assembled genomes (MAGs), showing that the enrichment culture primarily consisted of Gammaproteobacteria, Bacteroidota, Myxococcota, and Actinobacteriota. One MAG assigned to Rhodocuccus ruber strain C1 was the most active in the community, showing upregulation of various monooxygenase, dehydrogenase, and phosphoesterase genes throughout the degradation process, and thus was identified as the key degrader of TBOEP and the metabolites. Another MAG affiliated with Ottowia mainly contributed to TBOEP hydroxylation. Our results provided a comprehensive understanding of the bacterial TBOEP degradation at community level. ? 2023 |
| WOS研究方向 | Engineering, Environmental ; Environmental Sciences ; Water Resources |
| 语种 | 英语 |
| WOS记录号 | WOS:000949642400001 |
| 源URL | [http://ir.gig.ac.cn/handle/344008/80325]  |
| 专题 | 有机地球化学国家重点实验室 |
| 作者单位 | 1.University of the Chinese Academy of Sciences, Beijing; 100049, China 2.CAS Center for Excellence in Deep Earth Science, Guangzhou; 510640, China 3.State Key Laboratory of Organic Geochemistry, Guangdong Provincial Key Laboratory of Environmental Protection and Resources Utilization, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou; 510640, China |
| 推荐引用方式 GB/T 7714 | Liang, Yi,Zhou, Xiangyu,Wu, Yiding,et al. Meta-omics elucidates key degraders in a bacterial tris(2-butoxyethyl) phosphate (TBOEP)-degrading enrichment culture[J]. WATER RESEARCH,2023,233:119774. |
| APA | Liang, Yi.,Zhou, Xiangyu.,Wu, Yiding.,Wu, Yang.,Zeng, Xiangying.,...&Peng, Ping'an.(2023).Meta-omics elucidates key degraders in a bacterial tris(2-butoxyethyl) phosphate (TBOEP)-degrading enrichment culture.WATER RESEARCH,233,119774. |
| MLA | Liang, Yi,et al."Meta-omics elucidates key degraders in a bacterial tris(2-butoxyethyl) phosphate (TBOEP)-degrading enrichment culture".WATER RESEARCH 233(2023):119774. |
入库方式： OAI收割
来源：广州地球化学研究所
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