Isolation and Biosynthesis of Phenazine-Polyketide Hybrids from Streptomyces sp. KIB-H483
文献类型：期刊论文
| 作者 | Wang, Zhiyan; Yang, Feng-Xian; Liu, Chongxi; Wang, Li ; Qi, Yuxin; Cao, Minghang; Guo, Xiaowei; Li, Jie; Huang, Xueshuang; Yang, Jing
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| 刊名 | JOURNAL OF NATURAL PRODUCTS
 |
| 出版日期 | 2022 |
| 卷号 | 85期号:5页码:1324-1331 |
| 关键词 | GENE-CLUSTER ANTIBIOTICS CLONING |
| DOI | 10.1021/acs.jnatprod.2c00067 |
| 英文摘要 | A phenazine-polyketide hybrid compound, nexphenazine A (1), was isolated from Streptomyces sp. KIB-H483. The bioinformatic analysis of the draft genome of the producing strain and gene inactivation experiments revealed that the biosynthesis of 1 involves a phenazine-polyketide hybrid gene cluster. The abolished production of 1 as well as the accumulation of shunt metabolites 4-7 in mutant strain Delta npzI revealed the key role of the npzI gene, which encodes an NAD(P)H-dependent ketoreductase, in nexphenazine biosynthesis. The structures and absolute configurations of the isolated intermediates were established on the basis of spectroscopic data analysis, singlecrystal X-ray diffraction, chiral chromatography, and chemical conversion experiments. NpzI exhibited stereochemical selectivity in reducing the carbonyl group of 4. Nexphenazine biosynthesis is proposed to involve a condensation of the carboxyl group of phenazine with one molecule of methylmalonyl-CoA by a type I PKS, followed by a ketone reduction by NpzI and an unknown methylation reaction. |
| WOS记录号 | WOS:000806554600015 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/75665]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Zhiyan,Yang, Feng-Xian,Liu, Chongxi,et al. Isolation and Biosynthesis of Phenazine-Polyketide Hybrids from Streptomyces sp. KIB-H483[J]. JOURNAL OF NATURAL PRODUCTS,2022,85(5):1324-1331. |
| APA | Wang, Zhiyan.,Yang, Feng-Xian.,Liu, Chongxi.,Wang, Li.,Qi, Yuxin.,...&Huang, Sheng-Xiong.(2022).Isolation and Biosynthesis of Phenazine-Polyketide Hybrids from Streptomyces sp. KIB-H483.JOURNAL OF NATURAL PRODUCTS,85(5),1324-1331. |
| MLA | Wang, Zhiyan,et al."Isolation and Biosynthesis of Phenazine-Polyketide Hybrids from Streptomyces sp. KIB-H483".JOURNAL OF NATURAL PRODUCTS 85.5(2022):1324-1331. |
入库方式： OAI收割
来源：昆明植物研究所
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