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SARS-CoV-2 envelope protein-derived extracellular vesicles act as potential media for viral spillover

文献类型：期刊论文

作者Li, Shuangqu5,6; Bu, Jiwen4; Pan, Xiaoyan3; Li, Qiguang5,6; Zuo, Xiaoli6; Xiao, Gengfu3; Du, Jiulin4; Zhang, Lei-Ke2,3; Xia, Bingqing5,6; Gao, Zhaobing1,5,6
刊名JOURNAL OF MEDICAL VIROLOGY
出版日期2024-07-01
卷号96期号:7页码:11
关键词cellular signal transduction coronavirus cytokine/chemokine immune responses research and analysis methods virus classification
ISSN号0146-6615
DOI10.1002/jmv.29782
通讯作者Zhang, Lei-Ke(zhangleike@wh.iov.cn) ; Xia, Bingqing(xiabingqing@simm.ac.cn) ; Gao, Zhaobing(zbgao@simm.ac.cn)
英文摘要Extracellular vesicles (EVs) are shown to be a novel viral transmission model capable of increasing a virus's tropism. According to our earlier research, cells infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or transfected with envelope protein plasmids generate a novel type of EVs that are micrometer-sized and able to encase virus particles. Here, we showed the capacity of these EVs to invade various animals both in vitro and in vivo independent of the angiotensin-converting enzyme 2 receptor. First, via macropinocytosis, intact EVs produced from Vero E6 (monkey) cells were able to enter cells from a variety of animals, including cats, dogs, bats, hamsters, and minks, and vice versa. Second, when given to zebrafish with cutaneous wounds, the EVs showed favorable stability in aqueous environments and entered the fish. Moreover, infection of wild-type (WT) mice with heterogeneous EVs carrying SARS-CoV-2 particles led to a strong cytokine response and a notable amount of lung damage. Conversely, free viral particles did not infect WT mice. These results highlight the variety of processes behind viral transmission and cross-species evolution by indicating that EVs may be possible vehicles for SARS-CoV-2 spillover and raising risk concerns over EVs' potential for viral gene transfer.
WOS关键词EXOSOMES ; CELLS ; TRANSMISSION ; PATHWAY
资助项目National Science Fund of Distinguished Young Scholars[81825021] ; Fund of Youth Innovation Promotion Association[2019285] ; National Natural Science Foundation of China[31700732] ; National Natural Science Foundation of China[81773707] ; National Natural Science Foundation of China[92169202] ; National Key Research and Development Program[2020YFC0842000] ; National Key Laboratory Program[LG202101-01-04] ; Shanghai Science and Technology[20ZR1474200] ; Shanghai Science and Technology[22QA1411000]
WOS研究方向Virology
语种英语
WOS记录号WOS:001279358500001
出版者WILEY
源URL[http://119.78.100.183/handle/2S10ELR8/312691]  
专题中国科学院上海药物研究所
通讯作者Zhang, Lei-Ke; Xia, Bingqing; Gao, Zhaobing
作者单位1.Chinese Acad Sci, Inst Drug Discovery Innovat, Zhongshan Inst Drug Discovery, Zhongshan, Peoples R China
2.Hubei Jiangxia Lab, Wuhan, Hubei, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Virol, Ctr Biosafety Mega Sci, State Key Lab Virol, Wuhan, Hubei, Peoples R China
4.Chinese Acad Sci, Inst Neurosci, Ctr Excellence Brain Sci & Intelligence Technol, State Key Lab Neurosci, Shanghai, Peoples R China
5.Univ Chinese Acad Sci, Beijing, Peoples R China
6.Chinese Acad Sci, Shanghai Inst Mat Med, Stake Key Lab Drug Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Li, Shuangqu,Bu, Jiwen,Pan, Xiaoyan,et al. SARS-CoV-2 envelope protein-derived extracellular vesicles act as potential media for viral spillover[J]. JOURNAL OF MEDICAL VIROLOGY,2024,96(7):11.
APA Li, Shuangqu.,Bu, Jiwen.,Pan, Xiaoyan.,Li, Qiguang.,Zuo, Xiaoli.,...&Gao, Zhaobing.(2024).SARS-CoV-2 envelope protein-derived extracellular vesicles act as potential media for viral spillover.JOURNAL OF MEDICAL VIROLOGY,96(7),11.
MLA Li, Shuangqu,et al."SARS-CoV-2 envelope protein-derived extracellular vesicles act as potential media for viral spillover".JOURNAL OF MEDICAL VIROLOGY 96.7(2024):11.

入库方式： OAI收割

来源：上海药物研究所

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