Critical parameters to standardize the size and concentration determination of nanomaterials by nanoparticle tracking analysis
文献类型：期刊论文
| 作者 | Tian, Youxi1,2,3; Tian, Dong1; Peng, Xinsheng3; Qiu, Hong1,2 |
| 刊名 | INTERNATIONAL JOURNAL OF PHARMACEUTICS
 |
| 出版日期 | 2024-05-10 |
| 卷号 | 656页码:16 |
| 关键词 | Nanomaterials Nanoparticle Extracellular vesicle Sizing and counting nanoparticle tracking analysis (NTA) resistive pulse sensing (RPS) transmission electron microscopy (TEM) |
| ISSN号 | 0378-5173 |
| DOI | 10.1016/j.ijpharm.2024.124097 |
| 通讯作者 | Peng, Xinsheng(xspeng@gdmu.edu.cn) ; Qiu, Hong(hongqiu@simm.ac.cn) |
| 英文摘要 | The size and concentration are critical for the diagnostic and therapeutic applications of nanomaterials but the accurate measurement remains challenging. Nanoparticle tracking analysis (NTA) is widely used for size and concentration determination. However, highly repeatable standard operating procedures (SOPs) are absent. We adopted the "search-evaluate-test" strategy to standardize the measurement by searching the critical parameters. The particles per frame are linearly proportional to the sample concentration and the measured results are more accurate and repeatable when the concentration is 10(8)-10(9) particles/ml. The optimal detection threshold is around 5. The optimal camera level is such that it allows clear observation of particles without diffractive rings and overexposure. The optimal speed is <= 50 in AU and similar to 10 mu l/min in flow rate. We then evaluated the protocol using polydisperse polystyrene particles and we found that NTA could discriminate particles in bimodal mixtures with high size resolution but the performance on multimodal mixtures is not as good as that of resistive pulse sensing (RPS). We further analyzed the polystyrene particles, SiO2 particles, and biological samples by NTA following the SOPs. The size and concentration measured by NTA differentially varies to those determined by RPS and transmission electron microscopy. |
| WOS关键词 | ACCURATE PARTICLE-SIZE ; EXTRACELLULAR VESICLES ; SUBMICRON PARTICLES ; ANALYSIS NTA ; QUANTIFICATION ; NANOSIGHT ; PROTEINS |
| 资助项目 | Shanghai Pujiang Program[19PJ1411300] ; Guangdong Medical University[4SG21277P] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001287769300001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/312703]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Peng, Xinsheng; Qiu, Hong |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Carbohydrate Based Drug Res Ctr, 555 Zuchongzhi Rd, Shanghai 200031, Peoples R China 2.Univ Chinese Acad Sci, Sch Pharm, 19A Yuquan Rd, Beijing 100049, Peoples R China 3.Guangdong Med Univ, Sch Pharm, 1 City Ave Songshan Lake Sci & Tech Ind Pk, Dongguan 523808, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Youxi,Tian, Dong,Peng, Xinsheng,et al. Critical parameters to standardize the size and concentration determination of nanomaterials by nanoparticle tracking analysis[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2024,656:16. |
| APA | Tian, Youxi,Tian, Dong,Peng, Xinsheng,&Qiu, Hong.(2024).Critical parameters to standardize the size and concentration determination of nanomaterials by nanoparticle tracking analysis.INTERNATIONAL JOURNAL OF PHARMACEUTICS,656,16. |
| MLA | Tian, Youxi,et al."Critical parameters to standardize the size and concentration determination of nanomaterials by nanoparticle tracking analysis".INTERNATIONAL JOURNAL OF PHARMACEUTICS 656(2024):16. |
入库方式： OAI收割
来源：上海药物研究所
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