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Protein-encapsulated long-wavelength fluorescent probe hybrid for imaging lipid droplets in living cells and mice with non-alcoholic fatty liver

文献类型：期刊论文

作者Wang, Han-Min3,10; Li, Yan-Chen3,4,10; Sun, Lu-Lu9; Tang, Ming-Ye; Liu, Jia2,10; Cai, Jiahao4; Dong, Lei8; Li, Jia2,3,9,10; Zang, Yi1,3,4,10; Han, Hai-Hao3,9,10
刊名CHINESE CHEMICAL LETTERS
出版日期2024-11-01
卷号35期号:11页码:6
关键词Lipid droplets imaging Fluorescent probe Large Stokes shift Protein encapsulation Non-alcoholic fatty liver
ISSN号1001-8417
DOI10.1016/j.cclet.2024.109603
通讯作者Dong, Lei(leidong@wfmc.edu.cn) ; Li, Jia(jli@simm.ac.cn) ; Zang, Yi(yzang@lglab.ac.cn) ; Han, Hai-Hao(hanhaihao@simm.ac.cn) ; He, Xiao-Peng(xphe@ecust.edu.cn)
英文摘要Non-alcoholic fatty liver disease (NAFLD) is prevalent worldwide as a chronic liver disease that not only gives rise to hepatic complications, but leads to other chronic diseases such as type 2 diabetes and atherosclerosis. The aberrant accumulation of lipid droplets (LDs) in hepatocytes is a prominent signature of NAFLD. However, conventional techniques lack the capability to effectively monitor the dynamic changes in LD levels during NAFLD with living organisms. Hence, it is imperative to develop LD-specific long-wavelength fluorescent probes with high imaging contrast for the in-situ diagnosis of NAFLD. In this study, we synthesized a new LD-selective long-wavelength fluorescent probe, denoted as LD-1, , based on the twisted intramolecular charge transfer (TICT) mechanism. The probe exhibits a large Stokes shift and intensive fluorescence emission in nonpolar and viscous solutions. By self-assembling LD-1 with bovine serum albumin (BSA), a biocompatible, long-wavelength fluorescent probe hybrid, LD-1@BSA, , was formed, allowing for LDs to be selectively imaged in hepatocytes. Moreover, LD-1@BSA successfully discriminates NAFLD cells before and after drug treatment, and achieves non-invasive and real-time monitoring of LD accumulation in a mouse model of NAFLD. (c) 2024 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
WOS关键词HUMAN HEPATOCYTE ; NEUTRAL LIPIDS ; DIAGNOSIS ; DISEASE
资助项目National Natural Science Foundation of China[22107029] ; National Natural Science Foundation of China[22377135] ; National Natural Science Foundation of China[22305178] ; National Natural Science Foundation of China[92253306] ; National Natural Science Foundation of China[82130099] ; Taishan Scholars Program[tsqn202312305] ; Young Elite Scientists Sponsorship Program by Chinese Chemical Society, the Fundamental Research Projects of Science & Technology Innovation and development Plan in Yantai City[2023JCYJ059] ; Shandong Laboratory Program[SYS202205] ; Fundamental Research Funds for the Central Universities[222201717003] ; Programme of Introducing Talents of Discipline to Universities[B16017] ; Open Funding Project of the State Key Laboratory of Bioreactor Engineering ; State Key Laboratory of Drug Research[SKLDR-2023-KF-10] ; Ministry of Education Key Laboratory on signaling Regulation and Targeting Therapy of Liver Cancer (Naval Medical University)[2023-MEKLLC-MS/ZD-00*]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001288916700001
出版者ELSEVIER SCIENCE INC
源URL[http://119.78.100.183/handle/2S10ELR8/312718]  
专题中国科学院上海药物研究所
通讯作者Dong, Lei; Li, Jia; Zang, Yi; Han, Hai-Hao; He, Xiao-Peng
作者单位1.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China
2.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310058, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Lingang Lab, Shanghai 201203, Peoples R China
5.Eastern Hepatobiliary Surg Hosp, Natl Ctr Liver Canc, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China
6.East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Joint Int Res Lab Precis Chem, Shanghai 200237, Peoples R China
7.East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn,Key Lab Adv Mat, Shanghai 200237, Peoples R China
8.Weifang Med Univ, Sch Pharm, Weifang 261053, Peoples R China
9.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Peoples R China
10.Chinese Acad Sci, Shanghai Inst Mat Med, Mol Imaging Ctr, State Key Lab Chem Biol, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Han-Min,Li, Yan-Chen,Sun, Lu-Lu,et al. Protein-encapsulated long-wavelength fluorescent probe hybrid for imaging lipid droplets in living cells and mice with non-alcoholic fatty liver[J]. CHINESE CHEMICAL LETTERS,2024,35(11):6.
APA Wang, Han-Min.,Li, Yan-Chen.,Sun, Lu-Lu.,Tang, Ming-Ye.,Liu, Jia.,...&He, Xiao-Peng.(2024).Protein-encapsulated long-wavelength fluorescent probe hybrid for imaging lipid droplets in living cells and mice with non-alcoholic fatty liver.CHINESE CHEMICAL LETTERS,35(11),6.
MLA Wang, Han-Min,et al."Protein-encapsulated long-wavelength fluorescent probe hybrid for imaging lipid droplets in living cells and mice with non-alcoholic fatty liver".CHINESE CHEMICAL LETTERS 35.11(2024):6.

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来源：上海药物研究所

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