Positive-Charge-Based Small Molecule Dyes for Gut Microbiota Fluorescent Imaging
文献类型：期刊论文
| 作者 | Liu, Yue3,4; Wang, Zhiming2; Li, Ke3,4; Song, Ruihu2; Xu, Zhiqiang3,4; Yu, Tianhe3,4; Wang, Jing1; Feng, Xin3,4; Chen, Hao2 |
| 刊名 | ACS OMEGA
 |
| 出版日期 | 2024-08-19 |
| 卷号 | 9期号:34页码:36371-36379 |
| ISSN号 | 2470-1343 |
| DOI | 10.1021/acsomega.4c03727 |
| 通讯作者 | Feng, Xin(feng_xin@jlu.edu.cn) ; Chen, Hao(haoc@simm.ac.cn) |
| 英文摘要 | As one of the research hotspots in recent years, gut microbiota have been proven to be closely related to host metabolism, nutrient absorption, and immune regulation. However, there are still many urgent issues in the research of gut microbiota, such as the localization and tracking of gut microbiota. In this research, two new fluorescent probes, EF and 6F, were developed by optimizing the structure of the positron salt small molecule probe F16. In vitro labeling experiments showed that EF and 6F can quickly label Gram-positive bacteria, Staphylococcus aureus and Lactobacillus reuteri, as well as Gram-negative bacteria, Escherichia coli and Salmonella pullorum. Meanwhile, EF and 6F have little bacterial toxicity and are used at a maximum concentration of 200 mu M. Compared with EF, 6F has better hydrophilicity and stronger fluorescence characteristics in aqueous solutions, making it more suitable for imaging within gut microbiota populations. The results of in vivo imaging experiments indicate that EF and 6F can label and image the intestinal microbiota colonized by the mouse intestinal mucosal epithelium without causing any damage to intestinal tissue. Compared with commercially available MitoTracker dyes and fluorescein 5-isothiocyanate (FITC) dyes, EF and 6F exhibit better biocompatibility. Therefore, the compounds EF and 6F synthesized in this study are novel small molecule probes suitable for imaging gut microbiota, providing a better probe selection for exploring complex gut microbiota. |
| WOS关键词 | BACTERIA ; EXPRESSION |
| 资助项目 | Science and Technology Innovation Key R&D Program of Chongqing under[CSTB2023TIAD-STX0006] ; Science and Technology Commission of Shanghai Municipality[YDZX20233100004032001] ; National Key Research and Development Program of China[2023YFA1800804] ; National Science and Technology Innovation Major Project of China[2021ZD0203900] ; Lingang Laboratory[LG-QS-202206-01] ; National Natural Science Foundation of China[82071976] ; National Natural Science Foundation of China[JJKH20231204KJ] ; National Natural Science Foundation of China[82102110] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001294172200001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/312824]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Feng, Xin; Chen, Hao |
| 作者单位 | 1.First Hosp Jilin Univ, Radiol Dept, Changchun 130021, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Mol Imaging Ctr, State Key Lab Chem Biol, Shanghai 201203, Peoples R China 3.Jilin Univ, Coll Vet Med, Changchun 130062, Peoples R China 4.Jilin Univ, State Key Lab Diag & Treatment Severe Zoonot Infec, Changchun 130062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yue,Wang, Zhiming,Li, Ke,et al. Positive-Charge-Based Small Molecule Dyes for Gut Microbiota Fluorescent Imaging[J]. ACS OMEGA,2024,9(34):36371-36379. |
| APA | Liu, Yue.,Wang, Zhiming.,Li, Ke.,Song, Ruihu.,Xu, Zhiqiang.,...&Chen, Hao.(2024).Positive-Charge-Based Small Molecule Dyes for Gut Microbiota Fluorescent Imaging.ACS OMEGA,9(34),36371-36379. |
| MLA | Liu, Yue,et al."Positive-Charge-Based Small Molecule Dyes for Gut Microbiota Fluorescent Imaging".ACS OMEGA 9.34(2024):36371-36379. |
入库方式： OAI收割
来源：上海药物研究所
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