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Mass Spectrometry Imaging of Amino Acids Enabled by Quaternized Pyridinium Salt MALDI Probe

文献类型：期刊论文

作者Zhou, Hao12,13; Yuan, Jie12; Xu, Jianfeng10,11; Wang, Yang9; Xiong, Pei8,12; Zhao, Guode13; Jiang, Xianhuan6,7; Peng, Ying13; Ye, Yang4,5,6,7,8; Cheng, Gang3
刊名ANALYTICAL CHEMISTRY
出版日期2024-08-16
卷号96期号:34页码:13785-13791
ISSN号0003-2700
DOI10.1021/acs.analchem.4c01147
通讯作者Peng, Ying(yingpeng1999@163.com) ; Ye, Yang(yye@simm.ac.cn) ; Cheng, Gang(gangcheng@zcmu.edu.cn) ; Zheng, Jiang(zhengneu@yahoo.com) ; Liu, Jia(jia.liu@simm.ac.cn)
英文摘要The distribution of small biomolecules, particularly amino acids (AAs), differs between normal cells and cancer cells. Imaging this distribution is crucial for gaining a deeper understanding of their physiological and pathological significance. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) provides accurate in situ visualization information. However, the analysis of AAs remains challenging due to the background interference by conventional MALDI matrices. On tissue chemical derivatization (OTCD) MSI serves as an important approach to resolve this issue. We designed, synthesized, and tested a series of pyridinium salt probes and screened out the 1-(4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)phenyl)-2,4,6-triphenylpyridin-1-ium (DCT) probe with the highest reaction efficiency and the most effective detection. Moreover, a quantum chemistry calculation was executed to address mechanistic insight into the chemical nature of the novel probes. DCT was found to map 20 common AAs in normal mouse tissues for the first time, which allowed differentiation of AA distribution in normal, normal interstitium, tumor, and tumor interstitium regions and provided potential mechanistic insights for cancer research and other biomedical studies.
WOS关键词DERIVATIZATION ; METABOLISM ; IDENTIFICATION ; BRAIN
资助项目National Natural Science Foundation of China[82373827]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001293294400001
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/312827]  
专题新药研究国家重点实验室
通讯作者Peng, Ying; Ye, Yang; Cheng, Gang; Zheng, Jiang; Liu, Jia
作者单位1.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310058, Peoples R China
2.Guizhou Med Univ, State Key Lab Funct & Applicat Med Plants, Key Lab Pharmaceut Guizhou Prov, Guiyang 550025, Guizhou, Peoples R China
3.Zhejiang Chinese Med Univ, Sch Pharmaceut Sci, Hangzhou 310053, Peoples R China
4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Chinese Acad Sci, Shanghai Inst Mat Med, Nat Prod Chem Dept, Shanghai, Peoples R China
7.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
8.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China
9.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Liaoning, Peoples R China
10.Fudan Univ, Canc Metastasis Inst, Shanghai 201206, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Hao,Yuan, Jie,Xu, Jianfeng,et al. Mass Spectrometry Imaging of Amino Acids Enabled by Quaternized Pyridinium Salt MALDI Probe[J]. ANALYTICAL CHEMISTRY,2024,96(34):13785-13791.
APA Zhou, Hao.,Yuan, Jie.,Xu, Jianfeng.,Wang, Yang.,Xiong, Pei.,...&Liu, Jia.(2024).Mass Spectrometry Imaging of Amino Acids Enabled by Quaternized Pyridinium Salt MALDI Probe.ANALYTICAL CHEMISTRY,96(34),13785-13791.
MLA Zhou, Hao,et al."Mass Spectrometry Imaging of Amino Acids Enabled by Quaternized Pyridinium Salt MALDI Probe".ANALYTICAL CHEMISTRY 96.34(2024):13785-13791.

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来源：上海药物研究所

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