Multiomics insights into the TNT degradation mechanism by Pantoea sp. BJ2 isolated from an ammunition destruction site
文献类型：期刊论文
| 作者 | Li, You4; Luo, Junpeng3,4; Liao, Xiaoyong4; Cao, Hongying4; Pan, Junting2; James, Anina2; Li, Haonan4 |
| 刊名 | CHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2024-10-01 |
| 卷号 | 497页码:154957 |
| 关键词 | Pantoea sp. Biodegradation Trinitrotoluene Metabolic pathways Multiomics analysis |
| DOI | 10.1016/j.cej.2024.154957 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | Trinitrotoluene (TNT) is a highly toxic organic pollutant, and its biodegradation process and mechanism remain unclear. In this study, five TNT-degrading bacteria were isolated and identified, among which Pantoea sp. BJ2 showed the best TNT degradation potential; it could degrade 97.85 % of 100 mg L- 1 TNT within 24 h. Further, five genes (three nemA, one nfsA and one nfnB) directly related to TNT metabolism were identified in Pantoea sp. BJ2. During the degradation of TNT by Pantoea sp. BJ2, N-ethylmaleimide reductase, nitrate reductase and ATPbinding protein of the nitrate/nitrite transport system were found to be significantly upregulated, and five metabolites, including 2-amino-4,6-dinitrotoluene, were also identified. Based on the multiomics analysis, the mechanism of TNT degradation by Pantoea sp. BJ2 was proposed, indicating that the degradation of TNT by Pantoea sp. BJ2 was achieved under the joint action of various functional genes and proteins, including material transport, energy supply and exogenous material metabolism. The findings offer reliable theoretical underpinnings and technical support for a safe remediation of TNT-contaminated sites, providing new insights into the microbial degradation mechanisms of TNT from a multiomics perspective. |
| WOS关键词 | NITROAROMATIC COMPOUNDS ; 2,4,6-TRINITROTOLUENE ; BIOTRANSFORMATION ; BIODEGRADATION ; TOXICITY ; TRINITROTOLUENE ; NITROREDUCTASE ; TRANSFORMATION ; REDUCTION ; OXIDATION |
| WOS研究方向 | Engineering |
| WOS记录号 | WOS:001300050800001 |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/207919]  |
| 专题 | 陆地表层格局与模拟院重点实验室_外文论文 |
| 通讯作者 | Luo, Junpeng; Liao, Xiaoyong |
| 作者单位 | 1.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, State Key Lab Efficient Utilizat Arid & Semiarid A, Beijing 100081, Peoples R China 2.Henan Acad Sci, Inst Geog Sci, Zhengzhou 450052, Henan, Peoples R China 3.Beijing Key Lab Environm Damage Assessment & Remed, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Land Surface Pattern & Simulat, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, You,Luo, Junpeng,Liao, Xiaoyong,et al. Multiomics insights into the TNT degradation mechanism by Pantoea sp. BJ2 isolated from an ammunition destruction site[J]. CHEMICAL ENGINEERING JOURNAL,2024,497:154957. |
| APA | Li, You.,Luo, Junpeng.,Liao, Xiaoyong.,Cao, Hongying.,Pan, Junting.,...&Li, Haonan.(2024).Multiomics insights into the TNT degradation mechanism by Pantoea sp. BJ2 isolated from an ammunition destruction site.CHEMICAL ENGINEERING JOURNAL,497,154957. |
| MLA | Li, You,et al."Multiomics insights into the TNT degradation mechanism by Pantoea sp. BJ2 isolated from an ammunition destruction site".CHEMICAL ENGINEERING JOURNAL 497(2024):154957. |
入库方式： OAI收割
来源：地理科学与资源研究所
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