Systematic Characterization of Sesquiterpenes from Dendrobium nobile through Offline Two-Dimensional Chromatography Tandem Mass Spectrometry and Target Isolation
文献类型：期刊论文
| 作者 | Wang, Han-ze1,2; Li, Jia-yuan1; Yao, Chang-liang1; Li, Meng-meng1,2; Zhang, Yan1,2; Feng, Lin1,2; Guo, De-an1,2
|
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2024-08-27 |
| 卷号 | 72期号:36页码:19733-19747 |
| 关键词 | Dendrobium nobile sesquiterpenes systematiccharacterization target isolation |
| ISSN号 | 0021-8561 |
| DOI | 10.1021/acs.jafc.4c02305 |
| 通讯作者 | Guo, De-an(daguo@simm.ac.cn) |
| 英文摘要 | Dendrobium nobile is a species of the genus Dendrobium that can be used as both a medicinal herb and healthy food. The sesquiterpenes in D. nobile have attracted extensive attention in recent years. In this study, Amide x RP offline two-dimensional chromatography separation tandem high-resolution mass spectrometry combined with GNPS (Global Natural Product Social Molecular Networking) was developed for the characterization of sesquiterpenes in D. nobile. After first-dimensional amide separation, the 70% ethanol extract of D. nobile was divided into 40 fractions, which were analyzed by second-dimensional reverse-phase system separation and LTQ-Orbitrap detection. The raw data was imported into the GNPS, resulting in the efficient clustering of similar substances. Finally, 594 sesquiterpene compounds were characterized, and 25 compounds were isolated based on molecular network analysis, including six new compounds. In vitro bioassays, the isolated compounds decreased NO production in the LPS-induced microglial BV-2 cell model and the content of MDA in PC12 cells, demonstrating neuroprotective activity. These findings unraveled the underlying material and provided valuable insights into the quality control of D. nobile. |
| WOS关键词 | ALLOAROMADENDRANE ; PICROTOXANE ; DERIVATIVES ; GLYCOSIDES |
| 资助项目 | Qi-Huang Chief Scientist Project of the National Administration of Traditional Chinese Medicine ; National Natural Science Foundation of China[82130111] ; National Natural Science Foundation of China[32400325] |
| WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:001300212300001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/313038]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Guo, De-an |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Res Ctr TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China 2.Changchun Univ Chinese Med, Coll Pharm, Changchun 130117, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Han-ze,Li, Jia-yuan,Yao, Chang-liang,et al. Systematic Characterization of Sesquiterpenes from Dendrobium nobile through Offline Two-Dimensional Chromatography Tandem Mass Spectrometry and Target Isolation[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2024,72(36):19733-19747. |
| APA | Wang, Han-ze.,Li, Jia-yuan.,Yao, Chang-liang.,Li, Meng-meng.,Zhang, Yan.,...&Guo, De-an.(2024).Systematic Characterization of Sesquiterpenes from Dendrobium nobile through Offline Two-Dimensional Chromatography Tandem Mass Spectrometry and Target Isolation.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,72(36),19733-19747. |
| MLA | Wang, Han-ze,et al."Systematic Characterization of Sesquiterpenes from Dendrobium nobile through Offline Two-Dimensional Chromatography Tandem Mass Spectrometry and Target Isolation".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72.36(2024):19733-19747. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。