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Proanthocyanidins as the marker of amomum fruit from three botanical origins based on comprehensive profiling with LC-MS and GC-MS combined with chemometrics

文献类型：期刊论文

作者Deng, Kai2,3; Long, Huali3; He, Qingqing3; Hou, Jinjun3; Zhang, Zijia3; Zhou, Jing3; Zhou, Yang3; Shen, Minmin2; Yan, Qiang2; Wu, Wanying1,3
刊名PHYTOCHEMICAL ANALYSIS
出版日期2024-09-11
页码12
关键词amomum fruit feature-based molecular networking non-volatile components proanthocyanidins volatile components
ISSN号0958-0344
DOI10.1002/pca.3450
通讯作者Shen, Minmin(shenminmin_357@126.com) ; Yan, Qiang(yanqiangdoc@hotmail.com) ; Wu, Wanying(wanyingwu@simm.ac.cn)
英文摘要Introduction: Amomum fruit, also known as Sharen, serves as both a functional food and a traditional Chinese medicine with significant pharmacological activities. However, there are three botanical origins of Amomum fruit: Amomum villosum Lour. (AVL), Amomum villosum Lour. var. xanthioides T. L. (AVX), and Amomum longiligulare T. L. Wu (ALW). Objective: Conducting a comprehensive chemical composition analysis of Amomum fruit from three botanical origins aims to identify potential differences in metabolic characteristics. Methods: To annotate the metabolic characteristic ions of multi-origin Amomum fruit, we employed metabolomic techniques, including ultra-high-performance liquid chromatography (LC) coupled with linear ion trap-Orbitrap-tandem mass spectrometry (MS) and gas chromatography-MS, in conjunction with feature-based molecular networking technology. Additionally, chemometrics was utilized to examine the correlations between the various botanical origins. Results: A total of 201 non-volatile and 151 volatile metabolites were annotated, and most of the proanthocyanidins and flavonoids were identified by feature-based molecular networking. Additionally, 61 non-volatile and 45 volatile feature ions were screened out for classification. Principal component analysis, orthogonal projection to latent structures discrimination analysis, and heat map analysis were employed to clearly distinguish the metabolite profiles of Amomum fruit from different origins. Hierarchical clustering analysis indicated that proanthocyanidins C1 and C2, as well as proanthocyanins oligomers, show significant differential expression between AVX and AVL, which could be the new quality markers for the classification. Conclusion: Classification of the botanical origin of Amomum fruit based on LC-MS characteristic ions proved to be more advantageous. This study introduces new strategies and technical support for the quality control of Amomum fruit and facilitates the identification of unknown compounds for future research.
资助项目Public Welfare Technology Application Research Program of Huzhou ; Research Project of Science and Technology Commission of Shanghai Municipality[22DZ2204000] ; National Key R&D Program of China[2023YFC3504200] ; National Key R&D Program Strategic Scientific and Technological Innovation Cooperation Key Project[2022YFE0203600] ; National Key R&D Program Strategic Scientific and Technological Innovation Cooperation Key Project[SZZYSM202106004] ; [2022GZB03]
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry
语种英语
WOS记录号WOS:001310106000001
出版者WILEY
源URL[http://119.78.100.183/handle/2S10ELR8/313305]  
专题中国科学院上海药物研究所
通讯作者Shen, Minmin; Yan, Qiang; Wu, Wanying
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Zhejiang Chinese Med Univ, Huzhou Cent Hosp, Sch Clin Med 5, Huzhou 313003, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Res Ctr TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Deng, Kai,Long, Huali,He, Qingqing,et al. Proanthocyanidins as the marker of amomum fruit from three botanical origins based on comprehensive profiling with LC-MS and GC-MS combined with chemometrics[J]. PHYTOCHEMICAL ANALYSIS,2024:12.
APA Deng, Kai.,Long, Huali.,He, Qingqing.,Hou, Jinjun.,Zhang, Zijia.,...&Wu, Wanying.(2024).Proanthocyanidins as the marker of amomum fruit from three botanical origins based on comprehensive profiling with LC-MS and GC-MS combined with chemometrics.PHYTOCHEMICAL ANALYSIS,12.
MLA Deng, Kai,et al."Proanthocyanidins as the marker of amomum fruit from three botanical origins based on comprehensive profiling with LC-MS and GC-MS combined with chemometrics".PHYTOCHEMICAL ANALYSIS (2024):12.

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来源：上海药物研究所

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