Chemoenzymatic Labeling, Detection and Profiling of Core Fucosylation in Live Cells
文献类型：期刊论文
| 作者 | Zhu, Qiang6; Chaubard, Jean-Luc5; Geng, Didi6; Shen, Jiechen4; Ban, Lan5; Cheung, Sheldon T.5; Wei, Fangyu3; Liu, Yating3; Sun, Haofan2; Calderon, Angie1 |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2024-09-16 |
| 卷号 | 146期号:38页码:26408-26415 |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/jacs.4c09303 |
| 通讯作者 | Yi, Wen(wyi@zju.edu.cn) ; Hsieh-Wilson, Linda C.(lhw@caltech.edu) |
| 英文摘要 | Core fucosylation, the attachment of an alpha-1,6-linked-fucose to the N-glycan core pentasaccharide, is an abundant protein modification that plays critical roles in various biological processes such as cell signaling, B cell development, antibody-dependent cellular cytotoxicity, and oncogenesis. However, the tools currently used to detect core fucosylation suffer from poor specificity, exhibiting cross-reactivity against all types of fucosylation. Herein we report the development of a new chemoenzymatic strategy for the rapid and selective detection of core fucosylated glycans. This approach employs a galactosyltransferase enzyme identified fromCaenorhabditis elegansthat specifically transfers an azido-appended galactose residue onto core fucose via a beta-1,4 glycosidic linkage. We demonstrate that the approach exhibits superior specificity toward core fucose on a variety of complex N-glycans. The method enables detection of core fucosylated glycoproteins from complex cell lysates, as well as on live cell surfaces, and it can be integrated into a diagnostic platform to profile protein-specific core fucosylation levels. This chemoenzymatic labeling approach offers a new strategy for the identification of disease biomarkers and will allow researchers to further characterize the fundamental role of this important glycan in normal and disease physiology. |
| WOS关键词 | FUCOSE ; GLYCOSYLATION ; CANCER ; SPECIFICITY ; MECHANISMS ; DYNAMICS ; LECTINS ; PROBES ; FUT8 |
| 资助项目 | National Institutes of Health[R01 AG060540] ; National Institutes of Health[AG062324] ; National Natural Science Foundation of China (NSFC)[22325704] ; National Natural Science Foundation of China (NSFC)[32271331] ; National Natural Science Foundation of China (NSFC)[92353303] ; National Natural Science Foundation of China (NSFC)[32201045] ; National Natural Science Foundation of China (NSFC)[32471327] ; National Natural Science Foundation of China (NSFC)[22007092] ; Lingang Laboratory[LG-QS-202206-08] ; Fundamental Research Funds for Central Universities[K20220228] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001314297700001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/313323]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yi, Wen; Hsieh-Wilson, Linda C. |
| 作者单位 | 1.Southern Univ Sci & Technol, Sch Med, Dept Pharmacol, Shenzhen 518055, Guangdong, Peoples R China 2.Natl Ctr Prot Sci Beijing, Beijing Inst Life, State Key Lab Proteom, Beijing 102206, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Carbohydrate Based Drug Res Ctr, Shanghai 201203, Peoples R China 4.Northwest Univ, Coll Life Sci, Xian 710069, Peoples R China 5.CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA 6.Zhejiang Univ, Coll Life Sci, Hangzhou 310012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Qiang,Chaubard, Jean-Luc,Geng, Didi,et al. Chemoenzymatic Labeling, Detection and Profiling of Core Fucosylation in Live Cells[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2024,146(38):26408-26415. |
| APA | Zhu, Qiang.,Chaubard, Jean-Luc.,Geng, Didi.,Shen, Jiechen.,Ban, Lan.,...&Hsieh-Wilson, Linda C..(2024).Chemoenzymatic Labeling, Detection and Profiling of Core Fucosylation in Live Cells.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,146(38),26408-26415. |
| MLA | Zhu, Qiang,et al."Chemoenzymatic Labeling, Detection and Profiling of Core Fucosylation in Live Cells".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 146.38(2024):26408-26415. |
入库方式： OAI收割
来源：上海药物研究所
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