中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 新药研究国家重点实验室
Enhanced cytosolic RNA delivery through early endosome fusion-mediated release via probiotic-derived lipopolysaccharide (LPS)-incorporated vesicles

文献类型：期刊论文

作者Nie, Di5,6; Lv, Yishan2,6; Gao, Duo5,6; Xu, Anqi5,6; Li, Qinyu4,6; Li, Jiaxin5,6; Lu, Xiang4,6; Wang, Bingqi5,6; Wang, Jie1,6; Liu, Chang5,6
刊名NANO TODAY
出版日期2024-12-01
卷号59页码:15
关键词Early endosomal escape SiRNA delivery Membrane fusion Outer membrane vesicle Lipopolysaccharide Colorectal cancer therapy
ISSN号1748-0132
DOI10.1016/j.nantod.2024.102480
通讯作者Yu, Miaorong(mryu@simm.ac.cn) ; Gan, Yong(ygan@simm.ac.cn)
英文摘要Achieving efficient and secure cytosolic delivery is crucial for RNA therapeutics. Presently, delivery systems predominantly attain cytosolic release through membrane rupture or destabilization of late endosomes and lysosomes. However, these approaches lead to restricted RNA release and undesirable cytotoxicity, ultimately diminishing therapeutic efficacy. Herein, we proposed an efficient strategy based on early endosome fusion- mediated release, employing probiotic-derived lipopolysaccharide (LPS)-incorporated vesicles to enhance RNA delivery. The LPS is derived from Escherichia coli Nissle 1917 (EcN) and has a high safety confirmed by the authoritative pyrogen test. The LPS-rich outer membrane vesicles (OMVs) and synthetic chimeric liposomes (LPS-Lips) are found capable of efficient cytosolic RNA delivery by using LPS to fuse with early endosomes, as evidenced by super-resolution and real-time imaging. The OMVs and LPS-Lips (containing 10 % and 30 % EcNderived LPS) exhibit enhanced ability to deliver functional BCL-xL siRNA, leading to more significant gene silencing and cell apoptosis in comparison to the commercial Lipofectamine 2000 and RNAiMAX groups. The in vivo results demonstrate their superior efficacy on inhibiting tumor growth and prolonged survival time with enhanced safety. These findings highlight the early endosome fusion strategy with facilitated release efficiency and safety, offering guidelines for the rational design of enhanced RNA delivery systems.
WOS关键词COLI NISSLE 1917 ; SIRNA ; ESCAPE ; LPS ; NANOPARTICLES ; COMBINATION ; MODULATION ; TRANSPORT ; SURVIVAL ; TARGET
资助项目National Science Fund of Distinguished Young Scholars, China[82025032] ; National Science Fund of Distinguished Young Scholars, China[82304329] ; National Natural Science Foundation of China, China[82073773] ; Open Competition to Select the Best Candidates Key Technology Program for Nucleic Acid Drugs of NCTIB, China[NCTIB2022HS01006] ; Key Research Program of Chinese Academy of Sciences, China[ZDBS-ZRKJZ-TLC005] ; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China[SIMM0220232001] ; Shanghai Action Plan for Science, Technology and Innovation, China[23HC1401200] ; Young Elite Scientists Sponsorship Program by CAST, China[2022QNRC001] ; Shanghai Post-doctoral Excellence Program, China[2022693]
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science
语种英语
WOS记录号WOS:001313438900001
出版者ELSEVIER SCI LTD
源URL[http://119.78.100.183/handle/2S10ELR8/313350]  
专题新药研究国家重点实验室
通讯作者Yu, Miaorong; Gan, Yong
作者单位1.Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai 201203, Peoples R China
2.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China
3.Natl Inst Food & Drug Control, NMPA Key Lab Qual Res & Evaluat Pharmaceut Excipie, Beijing 100050, Peoples R China
4.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Nie, Di,Lv, Yishan,Gao, Duo,et al. Enhanced cytosolic RNA delivery through early endosome fusion-mediated release via probiotic-derived lipopolysaccharide (LPS)-incorporated vesicles[J]. NANO TODAY,2024,59:15.
APA Nie, Di.,Lv, Yishan.,Gao, Duo.,Xu, Anqi.,Li, Qinyu.,...&Gan, Yong.(2024).Enhanced cytosolic RNA delivery through early endosome fusion-mediated release via probiotic-derived lipopolysaccharide (LPS)-incorporated vesicles.NANO TODAY,59,15.
MLA Nie, Di,et al."Enhanced cytosolic RNA delivery through early endosome fusion-mediated release via probiotic-derived lipopolysaccharide (LPS)-incorporated vesicles".NANO TODAY 59(2024):15.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。