Natriuretic peptide receptor-C perturbs mitochondrial respiration in white adipose tissue
文献类型：期刊论文
| 作者 | Li, Shi-Jin2; Wei, Jin-Qiu3; Kang, Yuan-Yuan3; Wang, Rui-Qi1,3; Rong, Wu-Wei3; Zhao, Jia-Jia3; Deng, Qian-Wan3; Gao, Ping-Jin3; Li, Xiao-Dong3; Wang, Ji-Guang3 |
| 刊名 | JOURNAL OF LIPID RESEARCH
 |
| 出版日期 | 2024-09-01 |
| 卷号 | 65期号:9页码:17 |
| 关键词 | browning mitochondrial complex natriuretic peptide receptor C obesity white adipose tissue |
| ISSN号 | 0022-2275 |
| DOI | 10.1016/j.jlr.2024.100623 |
| 通讯作者 | Li, Xiao-Dong(flylxd@163.com) |
| 英文摘要 | Natriuretic peptide receptor-C (NPR-C) is highly expressed in adipose tissues and regulates obesity-related diseases; however, the detailed mechanism remains unknown. In this research, we aimed to explore the potential role of NPR-C in cold exposure and high-fat/high-sugar (HF/HS) diet-induced metabolic changes, especially in regulating white adipose tissue (WAT) mitochondrial function. Our findings showed that NPR-C expression, especially in epididymal WAT (eWAT), was reduced after cold exposure. Global Npr3 (gene encoding NPR-C protein) deficiency led to reduced body weight, increased WAT browning, thermogenesis, and enhanced expression of genes related to mitochondrial biogenesis. RNA-sequencing of eWAT showed that Npr3 deficiency enhanced the expression of mitochondrial respiratory chain complex genes and promoted mitochondrial oxidative phosphorylation in response to cold exposure. In addition, Npr3 KO mice were able to resist obesity induced by HF/HS diet. Npr3 knockdown in stromal vascular fraction (SVF)-induced white adipocytes promoted the expression of proliferator-activated receptor gamma coactivator 1 alpha (PGC1 alpha), uncoupling protein one (UCP1), and mitochondrial respiratory chain complexes. Mechanistically, NPR-C inhibited cGMP and calcium signaling in an NPR-B-dependent manner but suppressed cAMP signaling in an NPR-B-independent manner. Moreover, Npr3 knockdown induced browning via AKT and p38 pathway activation, which were attenuated by Npr2 knockdown. Importantly, treatment with the NPR-C-specific antagonist, AP-811, decreased WAT mass and increased PGC-1 alpha, UCP1, and mitochondrial complex expression. Our findings reveal that NPR-C deficiency enhances mitochondrial function and energy expenditure in white adipose tissue, contributing to improved metabolic health and resistance to obesity. |
| WOS关键词 | FAT-CELL ; OBESITY ; THERMOGENESIS ; ADIPOCYTES ; DISEASE ; INDUCE ; MOUSE ; HEART |
| 资助项目 | National Natural Science Foundation of China[82030006] ; National Natural Science Foundation of China[82070435] ; National Natural Science Foundation of China[82370426] ; National Key R&D Program of China[2022YFA1104200] ; Shanghai Municipal Commissions of Science and Technology[21ZR1454100] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001316792500001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/313454]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Xiao-Dong |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 2.Peking Univ, Inst Mol Med, Coll Future Technol, State Key Lab Membrane Biol, Beijing, Peoples R China 3.Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Dept Cardiovasc Med,State Key Lab Med Genom,Shangh, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shi-Jin,Wei, Jin-Qiu,Kang, Yuan-Yuan,et al. Natriuretic peptide receptor-C perturbs mitochondrial respiration in white adipose tissue[J]. JOURNAL OF LIPID RESEARCH,2024,65(9):17. |
| APA | Li, Shi-Jin.,Wei, Jin-Qiu.,Kang, Yuan-Yuan.,Wang, Rui-Qi.,Rong, Wu-Wei.,...&Wang, Ji-Guang.(2024).Natriuretic peptide receptor-C perturbs mitochondrial respiration in white adipose tissue.JOURNAL OF LIPID RESEARCH,65(9),17. |
| MLA | Li, Shi-Jin,et al."Natriuretic peptide receptor-C perturbs mitochondrial respiration in white adipose tissue".JOURNAL OF LIPID RESEARCH 65.9(2024):17. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。