Multi-step HPLC fractionation enabled in-depth and unbiased characterization of histone PTMs
文献类型：期刊论文
| 作者 | Zhao, Wensi7; Chen, Kaifeng3,4,5,6; Zhang, Jun5,6; Zhang, Mingya2,6; Guo, Jingli5,6; Xie, Dong7; Xu, Junyu1,6; Tan, Minjia1,5,6
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| 刊名 | JOURNAL OF CHROMATOGRAPHY A
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| 出版日期 | 2024-11-08 |
| 卷号 | 1736页码:10 |
| 关键词 | Histone PTM Histone mark Proteomics Post-translational modification |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2024.465368 |
| 通讯作者 | Xu, Junyu(jyxu@simm.ac.cn) ; Tan, Minjia(mjtan@simm.ac.cn) |
| 英文摘要 | Histone post-translational modifications (PTMs) are critical epigenetic regulatory factors. Histone PTMs are highly dynamic and complicated, encompassing over 30 structurally diverse modifications across nearly 180 amino acid residues, which generated extensive information regarding histone marks. In proteomics-based characterization of histone PTMs, chemical derivatization and antibody-based affinity enrichment were frequently utilized to improve the identification depth. However, chemical derivatization suffered from the occurrence of side reactions, and antibody-based affinity enrichment focused on specific PTM types of interest. In this research, we developed a multi-step fractionation strategy for comprehensively unbiased detection of histone PTM sites. By combining protein-level fractionation with peptide-level alkaline and acid phase fractionation, we developed the Multidimensional Fractionation based Histone Mark Identification Technology (MudFIT) and increased PTM identification to a total of 264 histone PTM sites. To the best of our knowledge, this strategy achieved the most comprehensive characterization of histone PTM sites in a single proteomics study. Using the same starting amount of sample, MudFIT identified more Kac sites and Kac peptides than those in antibody-based acetylated peptide enrichment. Moreover, in addition to well-studied histone marks, we discovered 36 potential new histone PTM sites including H2BK116bu, H4R45me2, H1K63pr, and uncovered unknown histone PTM types like aminoadipic on lysine and nitrosylation on tyrosine. Our data provided a method and resource for in-depth characterization of histone PTM sites, facilitating further biological understanding of histone marks. |
| WOS关键词 | PROPIONYLATION ; EXPRESSION ; CHROMATIN |
| 资助项目 | National Key Research and Devel-opment Program of China[2023YFA1800400] ; Program of Shanghai Academic Research Leader[22XD1420900] ; Innovative Research Team of High-Level Local Universities in Shanghai[SHSMU-ZDCX20212700] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001318242200001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/313466]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Xu, Junyu; Tan, Minjia |
| 作者单位 | 1.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China 2.Fudan Univ, Sch Pharm, Shanghai 201203, Peoples R China 3.Tongji Univ, Canc Ctr, Sch Med, Shanghai 200434, Peoples R China 4.Tongji Univ, Shanghai Peoples Hosp 4, Translat Res Inst Brain & Brain Like Intelligence, Shanghai 200434, Peoples R China 5.Univ Chinese Acad Sci, Beijing 101408, Peoples R China 6.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 7.Tongji Univ, Shanghai Pulm Hosp, Sch Med, Dept Thorac Surg, Shanghai 200433, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Wensi,Chen, Kaifeng,Zhang, Jun,et al. Multi-step HPLC fractionation enabled in-depth and unbiased characterization of histone PTMs[J]. JOURNAL OF CHROMATOGRAPHY A,2024,1736:10. |
| APA | Zhao, Wensi.,Chen, Kaifeng.,Zhang, Jun.,Zhang, Mingya.,Guo, Jingli.,...&Tan, Minjia.(2024).Multi-step HPLC fractionation enabled in-depth and unbiased characterization of histone PTMs.JOURNAL OF CHROMATOGRAPHY A,1736,10. |
| MLA | Zhao, Wensi,et al."Multi-step HPLC fractionation enabled in-depth and unbiased characterization of histone PTMs".JOURNAL OF CHROMATOGRAPHY A 1736(2024):10. |
入库方式： OAI收割
来源：上海药物研究所
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