Metagenomic 18S rDNA reads revealed zonation of eukaryotic communities in the Yongle blue hole
文献类型：期刊论文
| 作者 | Zhang, Hongxi4,5 ; Wei, Taoshu5; Li, Qingmei5; Fu, Liang3; Li, Manjie1,2; He, Lisheng5; Wang, Yong1,2
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| 刊名 | FRONTIERS IN MICROBIOLOGY
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| 出版日期 | 2024-07-29 |
| 卷号 | 15页码:13 |
| 关键词 | Yongle blue hole 18S rRNA gene eukaryotic communities anoxic water metagenomics |
| DOI | 10.3389/fmicb.2024.1420899 |
| 英文摘要 | The Yongle blue hole (YBH), situated in the South China Sea, represents a compelling subject of study in marine microbiology due to its unique redox-layered microbial ecosystems. However, the diversity and ecology of microbial eukaryotes within the YBH remains underexplored. This study endeavors to bridge this gap through the application of the in situ microbial filtration and fixation (ISMIFF) device to collect 0.22-30 mu m microbial samples from 21 water layers of YBH. Subsequent extraction of 18S rRNA metagenomic reads of 21 metagenomes and 10 metatranscriptomes facilitated a comprehensive analysis of community structures. Findings revealed a pronounced superiority in the diversity and richness of eukaryotic microorganisms in the oxic zone compared to its suboxic and anoxic counterparts. Notably, Dinophyceae and Maxillopoda emerged as the predominant taxa based on the analysis of the 18S rRNA reads for the V4 and V9 regions, which showed stratification In their relative abundance and suggested their potential role in the thermo-halocline boundaries and oxic-anoxic interface. Specifically, In these eukaryotic microbial communities, Dinophyceae exhibited significant abundance at 20 m (20.01%) and 105 m (26.13%) water depths, while Maxillopoda was prevalent at 40 m (22.84%), 80 m (23.19%), and 100 m (15.42%) depths. A part of these organisms, identified as larvae and protists, were likely attracted by swarming chemosynthetic bacterial prey prevailing at the thermo-halocline boundaries and oxic-anoxic interface. Furthermore, the phylogenetic relationships of the major 18S operational taxonomic units (OTUs) showed a close adjacency to known species, except for three Dinophyceae OTUs. In conclusion, this study provides critical insights into the vertical distribution and transcriptional activity of <30-mu m eukaryotic microbes, shedding light on the taxonomic novelty of eukaryotic microorganisms within the semi-enclosed blue holes. |
| WOS关键词 | O-2/H2S GRADIENT ; DIVERSITY ; FJORD ; OCEAN ; CHEMOCLINE ; FRAMVAREN ; COPEPODA ; CARBON ; CAVE |
| 资助项目 | National Natural Science Foundation of China10.13039/501100001809 |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001289438000001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | National Natural Science Foundation of China10.13039/501100001809 |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/11463]  |
| 专题 | 研究生部 深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组 |
| 通讯作者 | Wang, Yong |
| 作者单位 | 1.Tsinghua Univ, Tsinghua Shenzhen Int Grad Sch, Shenzhen Key Lab Adv Technol Marine Ecol, Shenzhen 518055, Peoples R China 2.Shenzhen Int Grad Sch, Inst Ocean Engn, Shenzhen, Peoples R China 3.Sansha Track Ocean Coral Reef Conservat Res Inst C, Sansha, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Hongxi,Wei, Taoshu,Li, Qingmei,et al. Metagenomic 18S rDNA reads revealed zonation of eukaryotic communities in the Yongle blue hole[J]. FRONTIERS IN MICROBIOLOGY,2024,15:13. |
| APA | Zhang, Hongxi.,Wei, Taoshu.,Li, Qingmei.,Fu, Liang.,Li, Manjie.,...&Wang, Yong.(2024).Metagenomic 18S rDNA reads revealed zonation of eukaryotic communities in the Yongle blue hole.FRONTIERS IN MICROBIOLOGY,15,13. |
| MLA | Zhang, Hongxi,et al."Metagenomic 18S rDNA reads revealed zonation of eukaryotic communities in the Yongle blue hole".FRONTIERS IN MICROBIOLOGY 15(2024):13. |
入库方式： OAI收割
来源：深海科学与工程研究所
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