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A Nanofluorescent Probe for Evaluating the Fluctuation of Aminopeptidase N in Nonalcoholic Fatty Liver Disease and Hepatic Fibrosis

文献类型：期刊论文

作者Sun, Xiao3; Wang, Xinlei3; Fu, Lili1,4; Wang, Xiaoyan3,4; Chen, Lingxin2,3,4; Huang, Yan3
刊名ANALYTICAL CHEMISTRY
出版日期2024-08-29
卷号96期号:36页码:14639-14649
ISSN号0003-2700
DOI10.1021/acs.analchem.4c03298
通讯作者Chen, Lingxin(lxchen@yic.ac.cn) ; Huang, Yan(yhuang@yic.ac.cn)
英文摘要Aminopeptidase N (APN/CD13) is a widely expressed transmembrane ectoenzyme that is crucial for maintaining normal physiological activities. It exhibits abnormal activity closely associated with hepatic fibrosis and nonalcoholic fatty liver disease (NAFLD). Therefore, there is a high demand for noninvasive detection of aminopeptidase N (APN) in the diagnosis and research of related diseases. Here, we developed a small molecule fluorescent probe, Hcy-APN, which is a fluorescent probe with high sensitivity and selectivity for the detection of APN. Furthermore, we synthesized the fluorescent nanoprobe Hcy-APN@MSN by self-assembling Hcy-APN and mesoporous silica nanoparticles in solution using a combination of molecular probe design and nanofunctionalization strategies. The detection limit of this probe was 1.5 ng/mL. Hcy-APN@MSN exhibits more stable spectral characteristics compared to Hcy-APN and is suitable for detecting APN activity in live cells and mice. Hcy-APN@MSN was utilized for in vivo and intracellular imaging of NAFLD and hepatic fibrosis at different stages, as well as for a systematic assessment of APN levels in the liver. The results confirm an elevation in the expression levels of APN in NAFLD and hepatic fibrosis models. Furthermore, we investigated the inhibitory effect of the APN inhibitor bestatin in nonalcoholic fatty liver and hepatic fibrosis disease models, confirming its regulatory effect on APN levels in cells and in vivo in both disease models. Therefore, this study may offer diagnostic possibilities for detecting NAFLD and hepatic fibrosis.
WOS关键词CLINICAL-SIGNIFICANCE ; THERAPEUTIC TARGET ; CD13 ; PREVALENCE ; MANAGEMENT ; BESTATIN ; CELLS
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001302851000001
资助机构National Natural Science Foundation of China ; Research Initiation Fund of Binzhou Medical University ; Taishan Scholar Project Special Funding
源URL[http://ir.yic.ac.cn/handle/133337/35773]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_山东省海岸带环境工程技术研究中心
通讯作者Chen, Lingxin; Huang, Yan
作者单位1.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Peoples R China
2.Shaoxing Univ, Coll Chem & Chem Engn, Shaoxing 312000, Peoples R China
3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Xiao,Wang, Xinlei,Fu, Lili,et al. A Nanofluorescent Probe for Evaluating the Fluctuation of Aminopeptidase N in Nonalcoholic Fatty Liver Disease and Hepatic Fibrosis[J]. ANALYTICAL CHEMISTRY,2024,96(36):14639-14649.
APA Sun, Xiao,Wang, Xinlei,Fu, Lili,Wang, Xiaoyan,Chen, Lingxin,&Huang, Yan.(2024).A Nanofluorescent Probe for Evaluating the Fluctuation of Aminopeptidase N in Nonalcoholic Fatty Liver Disease and Hepatic Fibrosis.ANALYTICAL CHEMISTRY,96(36),14639-14649.
MLA Sun, Xiao,et al."A Nanofluorescent Probe for Evaluating the Fluctuation of Aminopeptidase N in Nonalcoholic Fatty Liver Disease and Hepatic Fibrosis".ANALYTICAL CHEMISTRY 96.36(2024):14639-14649.

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来源：烟台海岸带研究所

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