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Peptide-Driven Assembly of Magnetic Beads for Potentiometric Sensing of Bacterial Enzyme at a Subcellular Level

文献类型：期刊论文

作者Zhang, Han2,3; Mou, Junsong2,3; Ding, Jiawang2,3; Qin, Wei1,2,3,4
刊名ACS SENSORS
出版日期2024-08-24
页码9
关键词potentiometric sensing subcellular enzyme peptide assembly bacteria alkaline phosphatase
ISSN号2379-3694
DOI10.1021/acssensors.4c01632
通讯作者Ding, Jiawang(jwding@yic.ac.cn)
英文摘要Bacterial enzymes with different subcellular localizations play a critical ecological role in biogeochemical processing. However, precisely quantifying enzymes localized at certain subcellular levels, such as extracellular enzymes, has not yet been fully realized due to the complexity and dynamism of the bacterial outer membrane. Here we present a magneto-controlled potentiometric sensing platform for the specific detection of extracellular enzymatic activity. Alkaline phosphatase (ALP), which is one of the crucial hydrolytic enzymes in the ocean, was selected as the target enzyme. Magnetic beads functionalized with an ALP-responsive self-assembled peptide (GGGGGFFFpYpYEEE, MBs-peptides) prevent negatively charged peptides from entering the bacterial outer membrane, thereby enabling direct potentiometric sensing of extracellular ALP both attached to the bacterial cell surface and released into the surrounding environment. The dephosphorylation-triggered assembly of peptide-coupled magnetic beads can be directly and sensitively measured by using a magneto-controlled sensor. In this study, extracellular ALP activity of Pseudomonas aeruginosa at concentrations ranging from 10 to 1.0 x 10(5) CFU mL(-1) was specifically and sensitively monitored. Moreover, this magneto-controlled potentiometric method enabled a simple and accurate assay of ALP activity across different subcellular localizations.
WOS关键词ALKALINE-PHOSPHATASE ACTIVITY ; PHOSPHORUS ; COMPONENT
WOS研究方向Chemistry ; Science & Technology - Other Topics
语种英语
WOS记录号WOS:001298226700001
资助机构National Natural Science Foundation of China ; Taishan Scholar Program of Shandong Province ; Special Fund for the Scholar Program of Yantai and Shandong Province ; Key Development Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences ; Seed Project of Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences
源URL[http://ir.yic.ac.cn/handle/133337/35776]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_近岸生态与环境实验室
通讯作者Ding, Jiawang
作者单位1.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Han,Mou, Junsong,Ding, Jiawang,et al. Peptide-Driven Assembly of Magnetic Beads for Potentiometric Sensing of Bacterial Enzyme at a Subcellular Level[J]. ACS SENSORS,2024:9.
APA Zhang, Han,Mou, Junsong,Ding, Jiawang,&Qin, Wei.(2024).Peptide-Driven Assembly of Magnetic Beads for Potentiometric Sensing of Bacterial Enzyme at a Subcellular Level.ACS SENSORS,9.
MLA Zhang, Han,et al."Peptide-Driven Assembly of Magnetic Beads for Potentiometric Sensing of Bacterial Enzyme at a Subcellular Level".ACS SENSORS (2024):9.

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来源：烟台海岸带研究所

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