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  4. 中科院海岸带环境过程与生态修复重点实验室
Evaluation of monoamine oxidase B fluctuation in liver fibrosis cell and mice models via a specificity fluorescent probe

文献类型：期刊论文

作者Sun, Mingzhao2; Huang, Yan2; Sun, Xiao2; Fu, Lili2,3; Wang, Lingxiao2; Wang, Xinlei2; Wang, Xiaoyan2,3; Chen, Lingxin1,2,3
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2024-10-15
卷号417页码:8
关键词Liver fibrosis Monoamine oxidase B Fluorescence probe In -situ monitoring Early diagnosis
DOI10.1016/j.snb.2024.136111
通讯作者Huang, Yan(yhuang@yic.ac.cn) ; Wang, Xiaoyan(wangxy@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要Liver fibrosis can result in cirrhosis and cancer, leading to damage to liver function and even endangering life. Early diagnosis of fibrosis is essential for optimizing the therapeutic schedule and improving the cure rates. Recent researches have shown that monoamine oxidase B (MAO-B) could be used as the marker for the early diacrisis of liver fibrosis. However, there still lacked strategies for accurate in situ monitoring of MAO-B. In this work, we developed a fluorescence probe YXHcy-NH2 for detecting MAO-B activity in living cells and in vivo. MAO-B activates the probe within 100 minutes and emits bright fluorescence at the fluorescence emission peak. The results displayed that the fluorescence intensity of fibrotic LX-2 cells was ten times that of normal cells, and the fluorescence brightness of liver tissues in liver fibrotic mice was five times higher than that of normal mice, so our probe could successfully distinguish between fibrotic cells and normal cells, as well as liver fibrotic mice and normal mice. Our study provides an easy, rapid, and high sensitive method for in situ in vivo imaging of MAO-B. It may become an effective mean for early diagnosis of liver fibrosis.
WOS关键词TRANSIENT ELASTOGRAPHY ; INHIBITOR ; DIAGNOSIS ; MAO ; PF9601N ; STRESS
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
WOS记录号WOS:001252775900001
资助机构National Nature Science Foundation of China ; Research Initiation Fund of Binzhou Medical University ; Taishan Scholar Project Special Funding ; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization
源URL[http://ir.yic.ac.cn/handle/133337/35908]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_山东省海岸带环境工程技术研究中心
通讯作者Huang, Yan; Wang, Xiaoyan; Chen, Lingxin
作者单位1.Shaoxing Univ, Coll Chem & Chem Engn, Shaoxing 312000, Peoples R China
2.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Mingzhao,Huang, Yan,Sun, Xiao,et al. Evaluation of monoamine oxidase B fluctuation in liver fibrosis cell and mice models via a specificity fluorescent probe[J]. SENSORS AND ACTUATORS B-CHEMICAL,2024,417:8.
APA Sun, Mingzhao.,Huang, Yan.,Sun, Xiao.,Fu, Lili.,Wang, Lingxiao.,...&Chen, Lingxin.(2024).Evaluation of monoamine oxidase B fluctuation in liver fibrosis cell and mice models via a specificity fluorescent probe.SENSORS AND ACTUATORS B-CHEMICAL,417,8.
MLA Sun, Mingzhao,et al."Evaluation of monoamine oxidase B fluctuation in liver fibrosis cell and mice models via a specificity fluorescent probe".SENSORS AND ACTUATORS B-CHEMICAL 417(2024):8.

入库方式： OAI收割

来源：烟台海岸带研究所

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